Abstract: This study was conducted to identify polymorphisms of the Nangyang cattle’s growth hormone (GH) and insulin-like growth factor-I (IGF-I) and insulin-like growth factor-I binding protein 3 gene by the single nucleotide Polymorphisms and Polymerase chain reaction-based restriction fragment length polymorphism and to study association of polymorphisms identified in these genes with growth traits of the birth, 6 month, 12 month, 18 month, 24 month and 36 month. Results from the analysis showed a significant association of the BB genotype in the promoter of GH gene (GH-P5) with higher body length and body height during from 6month to 18month. From 24month to 36 month, the IGF-I-BP3 locus has a dominance modulation and control effect on backbody in Nanyang Cattle, and the BB genotype with higher Rump width than AA.