中国美利奴(新疆军垦型)绵羊9个微卫星基因座多态性研究

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HEREDITAS ›› 2006, Vol. 28 ›› Issue (8): 939-944.

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Polymorphism Distributions of 9 Microsatellite Loci in Chinese Merino Sheep

ZHAO Zong-Sheng ^1,2, WANG Gen-Lin ¹, Guo Ji-Gang ², LI Da-Quan ²

1. College of Animal Science and Technology, Nanjing Agriculture University , Nanjing 210095, China; 2 .College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Received:2005-07-12 Revised:2006-01-25 Online:2006-08-10 Published:2006-08-10
Contact: WANG Gen-Lin

Abstract

Abstract:

The polymorphism distributions of nine microsatellite loci, BM6506， BM1824，BM6438, ILSTS004 and OarDB6 on Chinese merino sheep chromosome 1 and BM4621， OarHH55， BM143 and OarJMP8 in sheep chromosome 6 were determined by polymerase chain reaction (PCR) and multiplex gel electrophoresis followed by silver staining. Gene frequency (Pi), power of discrimination (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (PE) were calculated. All loci obeyed Hardy-Weinberg equilibrium. BM4621 displayed the highest DP, H, PIC and PE values among the nine microsatellite loci. Cumulative DP of the nine microsatellite loci is 0.99999 and cumulative PE is 0.99915. These results showed that the nine microsatellite loci could be used in linkage analysis, individual identification and paternity test in Chinese merino sheep.

CLC Number:

Q953

ZHAO Zong-Sheng, WANG Gen-Lin, Guo Ji-Gang, LI Da-Quan. Polymorphism Distributions of 9 Microsatellite Loci in Chinese Merino Sheep[J]. HEREDITAS, 2006, 28(8): 939-944.

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Polymorphism Distributions of 9 Microsatellite Loci in Chinese Merino Sheep

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