Abstract: 4 Microsatellite marks linked with E.coli F4 receptor gene were selected from the 13 chromosome in pig to analyze hereditary difference between the genotype and F4 receptor adhesive appearance. Results showed that the 4 Microsatellite marks were highly polymorphic and the heterozygosities were among 0.6117 and 0.7500,the PIC were over 0.5749.The allele frequency on swine breeds were difference, the more linked, the more different. On F4ab adhesive appearance of S0222，the difference among genotypes was significant in SZL breed. The AC genotype of SW458 was no adhesin appearance in SZL and LW breeds, so the SW458 was potential to be one genetic marker on E.coli F4 resistive gene.