Abstract: Multimedia teaching method has been widely adopted by the teachers in universities and it is helpful for improving the teaching effects of the courses. According to the characteristics of “Genetics” course and the experiences for the construction of software and multimedia teaching practice, the construction of the teaching software, the characteristics of the multimedia teaching, the collecting of the teaching materials, the compiling of the multimedia teaching book, the improving of the multimedia teaching effects and the teaching level of teachers, and the mobilizing for the enthusiasm of students are discussed in this paper.