Abstract:

Gene cry1Ab was transformed into Minghui 63 (Oryza sativa L.) via Agrobacterium and several homozygous transgenic lines were obtained. In this study, the copy numbers, Bt protein contents, insect resistance, and main agronomic traits of these lines were examined. All these lines had single-copy number of cry1Ab through Southern blotting analysis. The line T_1Ab-10 and its hybrid expressed the higher Cry1Ab proteins than any other lines and their corresponding hybrids when assayed by ELISA. After artificial infestation of first-instar YSB (Yellow stem borer) and natural affection of stemborers and leaffolders, the line T_1Ab-10 showed high insect-resistant to these insects. Field trial displayed that, under the pesticide-applying condition, there was no statistical difference between the negative plants of T_1Ab-10 and control cultivar Minghui 63. These results indicate that the transgenic line T_1Ab-10 has the potential for commercialization and gene stack-ing.