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HEREDITAS ›› 2008, Vol. 30 ›› Issue (10): 1379-1382.doi: 10.3724/SP.J.1005.2008.01379

• 技术与方法 • Previous Articles    

Improvement of the silver-stained technique of polyacrylamide gel electrophoresis

LIANG Hong-Wei1, 2; WANG Chang-Zhong1, 2, 3; LI Zhong1, 2: LUO Xiang-Zhong1, 2;
ZOU Gui-Wei1, 2, 3
  

  1. 1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China;
    2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
    3. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2008-03-28 Revised:2008-08-01 Online:2008-10-10 Published:2008-10-10
  • Contact: ZOU Gui-Wei Hong-Wei

Abstract:

Abstract: Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.