Abstract:

Retinoic X receptor-gamma (RXRG) gene was studied as a candidate gene for the twinning trait of bovine. A new SNP A1941G was detected by sequencing at 3′UTR. Different genotypes were determined in Luxi monotocous cows, Luxi twinning cows, Chinese Simmental cows, Angus cows and Simmental×Mongolia cows by restriction fragment length polymorphism (RFLP). The value of polymorphism information content indicated that this was a moderate polymorphism in Luxi monotocous cows and Luxi twinning cows. The c2 test indicated that the polymorphic locus in Luxi twinning cows did not fit Hardy-Weinberg equilibrium (P<0.05). The c2 test of associational analysis between genotypic distribution and twinning or monotocous trait in Luxi cows showed that the difference was very significant (P<0.01).