Abstract:

Twenty microsatellite loci on Chromosome 1 and Chromosome Z were selected from chicken genome for correlation analysis with traits at first laying in silkies. The 180 silkies were from two egg-laying lines of BM and BF. Genotyping of microsatellite loci was carried out by using fluorescein-labeled multiplex-PCR detection technology. The results showed that MCW0068 and MCW0200 on Chromosome 1 were significantly associated with egg weight at first laying (P<0.05), MCW0068 and MCW0208 on Chromosome 1 and MCW0128 on Chromosome Z were significantly associated with body weight at first laying (P<0.05), and MCW0154 on Chromosome Z was highly significantly associated with body weight at first laying (P<0.01).