Abstract:

Although high-resolution picture can be obtained using the conventional banding method of DNA in PAGE gel, this method has complicated steps and will take very long time. In this paper, we reported an improved banding method of DNA in PAGE gel. DNA development in PAGE gel and comparison were performed by using two methods, the conventional banding method and the improved banding method, respectively. And the results indicated that the improved method has higher contrast between DNA band and the background in PAGE gel. So this improved method has higher resolution than the conventional one. Compared with the conventional banding method, this improved method has fewer steps, less time took and fewer reagents used. This improved method has replaced the conventional banding method completely in our laboratory.