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HEREDITAS ›› 2011, Vol. 33 ›› Issue (4): 404-410.doi: 10.3724/SP.J.1005.2011.00404

• en • Previous Articles    

The quality control of 4C-clone screening assay

TIAN Jing, CHEN Na, ZHAO Zhi-Hu, CHEN Hui-Peng   

  1. Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China
  • Received:2011-01-21 Revised:2011-03-16 Online:2011-04-20 Published:2011-04-25
  • Contact: ZHAO Zhi-Hu;CHEN Hui-Peng E-mail:zhaozh@nic.bmi.ac.cn;ChenHP0909@163.com

Abstract: Circular chromosome conformation capture (4C) can be used to analyze the high-resolution interaction map of cis-regulatory elements in genome-wide studies. In this study, we optimized the condition of PCR with the mimical 4C sample in order to establish a specific and efficient assay, which was validated practically. This 4C-clone screening assay can be used as the quality control in the application of 4C assay.

Key words: circular chromosome conformation capture, chromosomal interaction, quality control, SOX2, PCR amplification