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HEREDITAS ›› 2012, Vol. 34 ›› Issue (10): 1298-1303.doi: 10.3724/SP.J.1005.2012.01298

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Differential expression analysis of proteins in neutrophils between clinical mastitis and healthy dairy cows

LIU Kai-Dong1,2, LIU Nan1, DU Li-Xin3, WEI Cai-Hong3, ZHANG Li3, LU Guo-Bin3, ZHAO Fu-Ping3, LIU Ji-Feng1   

  1. 1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China 2. Qingdao Institute of Animal Husbandry and Veterinary Medicine, Qingdao 266100, China 3. National Center for Molecular Genetics and Breeding of Animal, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2012-04-10 Revised:2012-05-30 Online:2012-10-20 Published:2012-10-25

Abstract: Neutrophils provide the first line of defense against invading pathogens and have been reported to play a key role in bovine mammary immune. To examine the differential expression of proteins in neutrophils between clinical mastitis and healthy dairy cows, a 2-dimensional electrophoresis gel map with high repeatability was constructed for bovine neutrophils. From this map, seven differentially expressed proteins were identified by MALDI-TOF MS which are believed to be involved in pathways such as cell metabolism, oxidative stress, and inflammatory reaction. The differentially expressed proteins identified in this study may provide the basis for bovine mastitis resistance breeding research.

Key words: dairy cows, neutrophils, two-dimensional electrophoresis, proteomics