[an error occurred while processing this directive]

HEREDITAS ›› 2013, Vol. 35 ›› Issue (11): 1274-1283.doi: 10.3724/SP.J.1005.2013.01274

Previous Articles     Next Articles

Recent advances in the techniques of protein-protein interaction study

Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations.   

  1. 1. Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; 
    2. Tibet Academy of Agricultural and Animal Hushandry sciences, Lhasa 850000, China
  • Received:2013-06-05 Revised:2013-08-11 Online:2013-11-20 Published:2013-10-25

Abstract:

Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations.

Key words: protein-protein interactions, yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer, surface plasmon resonance analysis