干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析

doi:10.3724/SP.J.1005.2013.00666

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (5): 666-672.doi: 10.3724/SP.J.1005.2013.00666

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Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress

ZHANG Yu-Ting^1,2,3, ZHOU De-Qun³, SU Yuan⁴,YU Ping^1,2, ZHOU Xiao-Gang^1,2,YAO Chun-Xin^1,2

1. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China 2. Key Laboratory of Agricultural Biotechnology of Yunnan Province, Kunming 650223, China 3. Environmental Science and Engineering Faculty, Kunming University of Science and Technology, Kunming 650093, China 4. Life Science and Technology Faculty, Kunming University, Kunming 650214, China

Received:2012-09-05 Revised:2013-02-02 Online:2013-05-20 Published:2013-05-25

Abstract

Abstract: It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research progress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by using two-dimensional gel electrophoresis during drought and normal processing conditions.There were 12 differentially expressed protein spots identified by Electro- phoresis and MALDI-TOF-TOF/MS analysis. The function classification of these proteins results that the potato varieties to drought tolerance could be improved through the protection of photosynthesis and mitochondria, regulation of the signal transduction induced under environmental stress and regulation of plant tissue N and C transport system, these proteins expression were increased under drought.The results showed that these proteins are the drought resistance associated pro-teins of potato variety in Ninglang 182.This study provided a theoretical basis of the molecular mechanism of improving drought tolerance in order to expound the potato drought resistance variety through multiple paths and the level regulation.

Key words: drought resistance variety, proteomics, drought stress, two-dimensional gel electrophoresis

CLC Number:

S532
Q503

ZHANG Yu-Ting ZHOU De-Qun SU Yuan YU Ping ZHOU Xiao-Gang YAO Chun-Xin. Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress[J]. HEREDITAS, 2013, 35(5): 666-672.

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Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress

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