Abstract:

Spotted-leaf mutants form spots in leaves or leaf sheaths under normal condition. The spotted-leaf phenotypes are similar to hypersensitive reaction of plants attacked by pathogen. Identification and characterization of the spotted-leaf mutants are helpful for understanding the mechanisms of resistance to plant diseases. Here, we identify two spotted-leaf mutants spl101 and spl102 from an EMS-treated elite japonica cultivar KYJ (Kuanyejing). spl101 and spl102 form serious spots at the late heading stage. Genetic analyses show that the spotted-leaf phenotypes of both spl101 and spl102 are caused by a single recessive mutation, respectively. By employing the Mutmap method, we reveal that both spl101 and spl102 contain mutations in the OsEDR1 gene. The spl101 mutation occurs in the 5°-splicing site of the 6 ^th intron of OsEDR1, which causes abnormal recognition of the 6 ^th intron and leads to the frameshift mutation. The spl102 mutant contains a mutation in the tenth exon of OsEDR1, resulting in an amino acid change from the phenylalanine (F) to the cysteine (C). OsEDR1 has been reported to regulate pathogen-resistant reaction, and loss of OsEDR1 function produces similar phenotypes to those of spl101 and spl102. Here, two newly identified alleles of OsEDR1 will be benefit for further understanding the molecular mechanisms of the OsEDR1 gene in disease resistance, and will be helpful for enriching the rice germplasm resources. In addition, our results also validate the effectiveness of the Mutmap method in cloning the candidate mutations.

Key words: rice spotted-leaf mutant, Mutmap, OsEDR1