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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (6): 625-635.doi: 10.16288/j.yczz.24-307

• Review • Previous Articles     Next Articles

Application and progress of proximity labeling technology in the study of protein-protein interactions

Xinru Xu1(), Benhua Qiu2,3(), Xiumin Yan1()   

  1. 1. Ministry of Education-Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
    2. Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2024-11-18 Revised:2024-12-23 Online:2025-06-20 Published:2025-01-10
  • Contact: Xiumin Yan E-mail:9894678@sjtu.edu.cn;qiubenhua2018@sibcb.ac.cn;yanx@shsmu.edu.cn
  • Supported by:
    National Natural Science Foundation of China(32470724)

Abstract:

Protein-protein interactions are essential to almost all biological processes within cells. In recent years, proximity labeling technology has emerged as a cutting-edge approach for studying protein interactions and subcellular compartment proteomics. This technique enables labeling and capture of neighboring proteins by introducing chemical tags in the vicinity of a target protein. When combined with mass spectrometry, proximity labeling facilitates the effective identification of protein-protein interactions and proteomic landscapes of subcellular compartments with high spatiotemporal resolution. Compared to traditional methods, proximity labeling offers superior resolution, sensitivity, and real-time tracking capabilities,making it a powerful tool for investigating protein functions, interactions, and spatial proteomics within cells. In this review, we provide a comprehensive overview of the principles, classifications, and applications of proximity labeling technology, aiming to offer researchers valuable insights into this innovative methodology and to promote its broader application in cell biology, proteomics, and the study of disease mechanisms.

Key words: proximity labeling technology, protein-protein interactions, spatial proteome