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HEREDITAS ›› 2011, Vol. 33 ›› Issue (4): 371-377.doi: 10.3724/SP.J.1005.2011.00371

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Analysis of expressed sequence tags (ESTs) in sika deer (Cervus nippon hortulorum) velvet tip tissue

HAO Li, LI He-Ping, YAN Li   

  1. College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China
  • Received:2010-09-09 Revised:2010-12-07 Online:2011-04-20 Published:2011-04-25
  • Contact: LI He-Ping E-mail:lihepinghrb2002@yahoo.com.cn

Abstract: A total of 906 high quality ESTs were obtained through random sequencing from velvet tip tissue cDNA library of Sika Deer (Cervus nippon hortulorum). Clustering of 906 ESTs resulted in a set of 701 unigenes comprising of 86 con-tigs and 615 singletons. BLAST analysis revealed that 580 unigenes (82.7% of all 701 unigenes) were matched to the known genes or putative genes in public databases, which could be classified into molecular function, biological process or cellular component according to the Gene Ontology (GO) classifications. Thirty-nine unigenes were identified to be growth and development-related genes. This study provides the data available for NCBI public database of the deer genome.

Key words: cDNA library, velvet, sika deer, expressed sequence tags (ESTs), Gene Ontology (GO)