茉莉酸诱导侧根形成缺陷突变体asa1-1抑制子(soa)的鉴定与遗传分析

doi:10.3724/SP.J.1005.2011.01003

[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (9): 1003-1010.doi: 10.3724/SP.J.1005.2011.01003

• en • Previous Articles Next Articles

Genetic screening and analysis of suppressors of asa1-1 (soa) defective in jasmonate-mediated lateral root formation in Arabidopsis

LI Yan-An^1,2, QI Lin-Lin², SUN Jia-Qiang², LIU Hong-Yu¹, LI Chuan-You²

1. Northeast Agricultural University, College of Horticulture, Harbin 150030, China 2. State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biol-ogy, Chinese Academy of Sciences, Beijing 100101, China

Received:2011-02-23 Revised:2011-03-26 Online:2011-09-20 Published:2011-09-25
Contact: Li Chuan－you E-mail:cyli@genetics.ac.cn

Abstract

Abstract: It has been shown that jasmonate modulates the lateral root development through crosstalk with auxin in Arabidopsis thaliana. Exogenous application of jasmonate stimulates lateral root formation in wild type but inhibits lateral root formation in asa1-1. Our previous work has demonstrated that the lateral root formation defect of asa1-1 is co-related with jasmonte effect on PIN2 protein levels. To further elucidate the molecular mechanisms underlying jasmonate-mediated reduction of plasma membrane (PM)-resident PIN2 abundance, we have conducted a genetic screen to identify suppressors of asa1-1 (soa), which showed lateral root formation in the presence of jasmonate. Here, we described the basic characterization of soa563 and soa856. We showed that both soa563 and soa856 displayed restored lateral root formation in response to exogenous jasmonate. In addition, jasmonate-induced PIN2:GFP reduction was blocked in these two mutants. Our on-going effort to identify genes defined by these mutants promise to shed new light on the understanding of the molecular mechanisms controlling jasmonate-mediated regulation of PIN2 protein trafficking and turnover.

Key words: Arabidopsis thaliana, jasmonate, auxin, PIN2, lateral root formation

LI Yan-An, QI Lin-Lin, SUN Jia-Jiang, LIU Hong-Yu, LI Chuan-You. Genetic screening and analysis of suppressors of asa1-1 (soa) defective in jasmonate-mediated lateral root formation in Arabidopsis[J]. HEREDITAS, 2011, 33(9): 1003-1010.

References

[1] Fukaki H, Tasaka M. Hormone interactions during lateral root formation. Plant Mol Biol, 2009, 69(4): 437-449.
[2] Nibau C, Gibbs DJ, Coates JC. Branching out in new directions: the control of root architecture by lateral root formation. New Phytol, 2008, 179(3): 595-614.
[3] Fukaki H, Okushima Y, Tasaka M. Auxin-mediated lateral root formation in higher plants. Int Rev Cytol, 2007, 256: 111-137.
[4] De Smet I, Vanneste S, Inzé D, Beeckman T. Lateral root initiation or the birth of a new meristem. Plant Mol Biol, 2006, 60(6): 871-887.
[5] Casimiro I, Beeckman T, Graham N, Bhalerao R, Zhang HM, Casero P, Sandberg G, Bennett MJ. Dissecting Arabidopsis lateral root development. Trends Plant Sci, 2003, 8(4): 165-171.
[6] Celenza JL Jr, Grisafi PL, Fink GR. A pathway for lateral root formation in Arabidopsis thaliana. Genes Dev, 1995, 9(17): 2131-2142.
[7] Himanen K, Boucheron E, Vanneste S, de Almeida Engler J, Inzé D, Beeckman T. Auxin-mediated cell cycle activation during early lateral root initiation. Plant Cell, 2002, 14(10): 2339-2351.
[8] Casimiro I, Marchant A, Bhalerao RP, Beeckman T, Dhooge S, Swarup R, Graham N, Inzé D, Sandberg G, Casero PJ, Bennett M. Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell, 2001, 13(4): 843-852.
[9] Michniewicz M, Zago MK, Abas L, Weijers D, Schweighofer A, Meskiene I, Heisler MG, Ohno C, Zhang J, Huang F, Schwab R, Weigel D, Meyerowitz EM, Luschnig C, Offringa R, Friml J. Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell, 2007, 130(6): 1044-1056.
[10] Tanaka H, Dhonukshe P, Brewer PB, Friml J. Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development. Cell Mol Life Sci, 2006, 63(23): 2738-2754.
[11] Friml J. Subcellular trafficking of PIN auxin efflux carriers in auxin transport. Eur J Cell Biol, 2010, 89(2-3): 231-235.
[12] Wasternack C. Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Annals of Botany, 2007, 100(4): 681-697.
[13] Koo AJK, Fulda M, Browse J, Ohlrogge JB. Identification of a plastid acyl-acyl carrier protein synthetase in Arabidopsis and its role in the activation and elongation of exogenous fatty acids. The Plant Journal : for Cell and Molecular Biology, 2005, 44(4): 620-632.
[14] Sun JQ, Xu YX, Ye SQ, Jiang HL, Chen Q, Liu F, Zhou WK, Chen R, Li XQ, Tietz O, Wu XY, Cohen JD, Palme K, Li CY. Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Plant Cell, 2009, 21(5): 1495-1511.
[15] Blilou I, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B. The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature, 2005, 433(7021): 39-44.
[16] Menda N, Semel Y, Peled D, Eshed Y, Zamir D. In silico screening of a saturated mutation library of tomato. Plant J, 2004, 38(5): 861-872.
[17] Osmont KS, Sibout R, Hardtke CS. Hidden branches: developments in root system architecture. Annu Rev Plant Biol, 2007, 58: 93-113.
[18] Aloni R, Aloni E, Langhans M, Ullrich CI. Role of cytokinin and auxin in shaping root architecture: regulating vascular differentiation, lateral root initiation, root apical dominance and root gravitropism. Ann Bot, 2006, 97(5): 883-893.
[19] De Smet I, Tetsumura T, De Rybel B, dit Frey NF, Laplaze L, Casimiro I, Swarup R, Naudts M, Vanneste S, Audenaert D, Inzé D, Bennett MJ, Beeckman T. Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis. Development, 2007, 134(4): 681-690.
[20] Laplaze L, Benkova E, Casi

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Genetic screening and analysis of suppressors of asa1-1 (soa) defective in jasmonate-mediated lateral root formation in Arabidopsis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	Min Cao, Tongda Xu. The molecular mechanism of apical hook development in dicot plant [J]. Hereditas(Beijing), 2021, 43(8): 723-736.
[2]	Di Wu, Linzhou Huang, Jin Gao, Yonghong Wang. The molecular mechanism of plant gravitropism [J]. HEREDITAS(Beijing), 2016, 38(7): 589-602.
[3]	Haiwei Shuai, Yongjie Meng, Xiaofeng Luo, Feng Chen, Ying Qi, Wenyu Yang, Kai Shu. The roles of auxin in seed dormancy and germination [J]. HEREDITAS(Beijing), 2016, 38(4): 314-322.
[4]	Huazhao Yuan, Mizhen Zhao, Weimin Wu, HongMei Yu, Yaming Qian, Zhuangwei Wang, Xicheng Wang. Genome-wide identification and expression analysis of auxin-related gene families in grape [J]. HEREDITAS(Beijing), 2015, 37(7): 720-730.
[5]	LIU Zhen-Hua, YU Yan-Chong, XIANG Feng-Ning. Auxin response factors and plant growth and development [J]. HEREDITAS, 2011, 33(12): 1335-1346.
[6]	FU Gan-Tang, TU Di-Qiu. Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses [J]. HEREDITAS, 2010, 32(8): 848-856.
[7]	ZHANG Gong-Yu, XU Pei-Zhou, YANG Hua, TUN Xian-Jun. Imprinting genes and it’s expression in Arabidopsis [J]. HEREDITAS, 2010, 32(7): 670-676.
[8]	XIE Chong-Bei, JIN Gu-Lei, XU Hai-Meng, SHU Jun. Construction and analysis of SOS pathway-related transcriptional regulatory network underlying salt stress response in Arabidopsis [J]. HEREDITAS, 2010, 32(6): 639-646.
[9]	WANG Wen-Guo, LI Dui, SHU Jia-Yi, WANG Qing-Hua, CHEN Fang. DNA methylation-mediated regulation of OsMAPK2 gene expression in rice callus formation [J]. HEREDITAS, 2010, 32(12): 1275-1280.