仔猪BPI基因表达水平与大肠杆菌F18菌株感染的关系

doi:10.3724/SP.J.1005.2011.01225

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (11): 1225-1230.doi: 10.3724/SP.J.1005.2011.01225

Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets

YE Lan¹, ZI Chen¹, LIU Lu¹, ZHU Jing¹, XIE Kai-Zhou¹, ZHU Guo-Qian^{2, 3, 4}, HUANG Xiao-Guo^{3, 4}, BAO Wen-Bin^{1, 3, 4}, WU Sheng-Long^{1, 3, 4}, WANG Jin-Yu¹

1. Animal Science and Technology College, Yangzhou University, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China 2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China 3. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China 4. Jiangsu (Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms, Yangzhou 225009, China

Received:2011-01-07 Revised:2011-05-11 Online:2011-11-20 Published:2011-11-25
Contact: WU Sheng-Long E-mail:slwu@yzu.edu.cn

Abstract

Abstract: Based on the established resource populations of Sutai pig, the expression of BPI gene was assayed by Real-time PCR to detect the tissue expression and analyze the differential expression between Escherichia coli F18-resistant and sensitive piglets. This study aimed at providing a theoretical foundation for further research on the role BPI gene in host immunity and resistance to E. coli F18. The results showed that the expression of BPI gene was extremely low or undetectable in tissues including heart, liver, spleen, lung, kidney, stomach, muscle, thymus, and lymph nodes, which was in a stark contrast to the significantly high levels in duodenum and jejunum. In the tissues of both jejunum and duodenum, the mRNA expression of BPI gene in resistant individuals was significant higher than that in the sensitive individuals (P<0.05). The results suggested that BPI gene was likely to be related to the intestinal infection caused by E. coli F18. It is possible that the increased expression of BPI gene in intestinal is in connection with the resistance to E. coli F18.

Key words: Escherichia coli F18, post-weaning piglets, BPI gene, Real-time PCR

YE Lan, ZI Chen, LIU Lu, ZHU Jing, XIE Kai-Zhou, ZHU Guo-Qian, HUANG Xiao-Guo, BAO Wen-Bin. Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets[J]. HEREDITAS, 2011, 33(11): 1225-1230.

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Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets

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