小鼠孤雌胚胎干细胞的建立及其向运动神经元分化的初探

doi:10.3724/SP.J.1005.2011.01231

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (11): 1231-1238.doi: 10.3724/SP.J.1005.2011.01231

Generation of mouse parthenogenetic embryonic stem cells and preliminary study of the differentiation ability to motor neurons

WANG Zhen-Dong^1,2, XUE Yuan¹, SHAN Zhi-Yan¹, ZHENG Zhong¹, LI Xue¹, WU Yan-Shuang¹, SUN Rui-Zhen¹, SHI Jian¹, LI Ming-Jie¹, LIU Zhong-Hua², LEI Lei¹

1. Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China 2. College of Life Science, Northeast Agricultural University, Harbin 150030, China

Received:2011-01-25 Revised:2011-04-09 Online:2011-11-20 Published:2011-11-25
Contact: LEI Lei E-mail:leil086@yahoo.com.cn

Abstract

Abstract: In this study, we generated embryonic stem cells from parthenogenetic embryos (PESCs), and induced them to differentiate to motor neurons, which could be an alternative source of histocompatible cells for replacement of therapy and theoretical foundation for studying the relationship of genome imprint and neural differentiation. The parthenogenetic activation rate of B6D2F1 mouse oocytes was 93.26%. We established eight parthenogenetic embryonic stem cell lines and the establishment rate from parthenogenetic embryos was 23.53%. The pluripotency marker Oct4, the cell surface marker SSEA-1, and alkaline phosphatase exhibited in PESCs. Karyotype analysis showed normal 40 chromosomes when examined at passages 10 and 30, which was in accordance with their oocyte origins. Three germinal layers were differentiated in vivo and in vitro. Mouse PESCs, which were treated by tretinoin and sonic hedgehog with extracellular matrix, could generate motor neurons that expressed the specific markers such as HB9 and Olig2.

Key words: Sonic hedgehog (Shh), motor neurons, differentiation, Tretinoin (RA), parthenogenetic embryonic stem cells, imprinted genes

WANG Zhen-Dong, XUE Yuan, SHAN Zhi-Yan, ZHENG Zhong, LI Xue, WU Yan-Shuang, SUN Rui-Zhen, SHI Jian, LEI Lei. Generation of mouse parthenogenetic embryonic stem cells and preliminary study of the differentiation ability to motor neurons[J]. HEREDITAS, 2011, 33(11): 1231-1238.

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Generation of mouse parthenogenetic embryonic stem cells and preliminary study of the differentiation ability to motor neurons

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