[an error occurred while processing this directive]

Hereditas(Beijing) ›› 2018, Vol. 40 ›› Issue (12): 1075-1091.doi: 10.16288/j.yczz.18-079

• Reviews • Previous Articles     Next Articles

Detection methods of genome editing in plants

Chunxia Liu,Lizhao Geng,Jianping Xu()   

  1. Syngenta Beijing Innovation Center, Beijing 102206, China
  • Received:2018-03-29 Revised:2018-08-18 Online:2018-12-20 Published:2018-09-06
  • Contact: Xu Jianping E-mail:jianping.xu@syngenta.com

Abstract:

The life science has entered a new chapter with the revolutionary implementation of the CRISPR/Cas9 genome editing technology in various living organisms. With the unique flexibility, feasibility and extendibility, the CRISPR/Cas9 technology greatly accelerates genetic engineering research, as well as plant molecular breeding. However, it has become a challenge to screen for and identify genome-edited plants at early stages in a rapid and high-throughput fashion, due to the massive number of plants produced from transformation process. In this review, we summarize the molecular methods developed in recent years to identify genome-edited plants. We compare their advantages and disadvantages, and the scope of application. In addition, we provide insights of the development trend of detection methods for plant genome editing. This review will serve as a reference for future genome editing research in plants.

Key words: non-homologous end joining (NHEJ), homologous recombination (HR), PCR/RE, mismatch cleavage assay, Sanger sequencing