体细胞克隆猪发育异常研究进展

doi:10.16288/j.yczz.20-105

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Hereditas(Beijing) ›› 2020, Vol. 42 ›› Issue (10): 993-1003.doi: 10.16288/j.yczz.20-105

• Review • Previous Articles Next Articles

Progress on abnormal development of cloned pigs generated by somatic cell transfer nuclear

Zheng Ao¹(), Xiang Chen¹, Zhenfang Wu², Zicong Li²

^1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China
^2. National Engineering Research Center for Swine Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received:2020-04-16 Revised:2020-07-05 Online:2020-10-20 Published:2020-07-29
Supported by:
Supported by the Scientific Research Project of Guizhou University Talents Fund (No. GDRJHZ-2019-21)

Abstract

Abstract:

Cloning, also known as somatic cell nuclear transfer (SCNT), is an asexual reproduction technique that reprograms differentiated cells to the totipotent state, and generates offspring with a genotype identical to the donor cells. Pig cloning technique holds great promise for propagating excellent breeding boars, generating genetically modified pigs, protecting rare and endangered pigs and studying the mechanisms of somatic cell nucleus reprogramming. However, cloned pigs suffer from various developmental defects, including low birth rate, low birth weight, and high stillbirth occurrence, neonatal mortality and congenital malformations, which severely hamper their applications. Errors in epigenetic reprogramming of donor nucleus are considered as the main causes of low cloning efficiency and abnormal embryonic development in cloned embryos and animals. However, most studies to correct the errors in epigenetic reprogramming of cloned pig embryos have not substantially improved the birth and survival rates of cloned pigs. In this review, we summarize the abnormal phenotypes, causes of abnormal development of cloned pigs and effective methods for improving pig cloning efficiency, thereby providing a reference for the future research to improve the development and survival rates of cloned pig embryos and cloned pigs.

Key words: SCNT, cloned pig, epigenetic reprogramming, abnormal development

Zheng Ao, Xiang Chen, Zhenfang Wu, Zicong Li. Progress on abnormal development of cloned pigs generated by somatic cell transfer nuclear[J]. Hereditas(Beijing), 2020, 42(10): 993-1003.

Figures/Tables 1

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代孕母猪数	移植胚胎数	怀孕率(%)	出生总仔数	活仔数	克隆效率(%)	参考文献
24	5288	54.2	58	46	1.10	[5]
66	11,911	21.2	40	—	0.34	[6]
8	904	75	19	15	2.10	[7]
265	62,090	21.5	65	51	0.10	[8]
11	2884	72.7	12	10	0.42	[9]
193	18,649	56.5	318	243	1.71	[10]
328	92,005	57.6	488	—	0.53	[11]
4	1342	75	15	5	1.12	[12]
656	228,230	62	1070	810	0.47	[13]
79	13,620	63.3	177	119	1.30	[14]
4	1187	50	6	6	0.51	[15]
5	530	100	11	—	2.07	[16]

Progress on abnormal development of cloned pigs generated by somatic cell transfer nuclear

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