膜锚定蛋白FvOPY2调控拟轮枝镰孢几丁质和伏马毒素合成与玉米穗腐致病性

doi:10.16288/j.yczz.26-068

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Hereditas(Beijing)

Membrane-anchored protein FvOPY2 regulates chitin and fumonisin biosynthesis and maize ear rot pathogenicity in Fusarium verticillioides

Xiuwu Jiang¹, Fengqin Wu¹, Yuetian Qian¹, Yanzhi Shao¹, Canxing Duan²

1. Chun'an County Center for Disease Control and Prevention, Chun'an County Health Supervision Institute, Zhejiang Province, Hangzhou 311700, China

2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2026-05-06 Revised:2026-08-10 Published:2026-08-26

Abstract

Abstract:

OPY2 is a highly conserved membrane signal-sensing protein in fungi, yet its function in Fusarium verticillioides, the causal agent of maize ear rot, remains unclear. In this study, we generated an FvOPY2 knockout mutant and found that FvOPY2 localizes to the plasma membrane and intracellular vesicles. FvOPY2 deletion caused reduced growth, sporulation defects, hypersensitivity to cell wall stressors, and significantly decreased fumonisin production and maize ear pathogenicity. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) revealed that FvOPY2 regulated the expression of the chitin synthase gene family and the fumonisin biosynthetic gene cluster. These findings demonstrate that FvOPY2 regulates chitin and fumonisin biosynthesis and maize ear infection, potentially acting as a signal receptor for environmental sensing. This study provides a theoretical basis and novel strategies for developing FvOPY2 as a target for new fungicides.

Key words:

Fusarium , verticillioides, membrane anchoring protein, chitin synthesis, fumonisin, , pathogenicity

Xiuwu Jiang, Fengqin Wu, Yuetian Qian, Yanzhi Shao, Canxing Duan.

Membrane-anchored protein FvOPY2 regulates chitin and fumonisin biosynthesis and maize ear rot pathogenicity in Fusarium verticillioides [J]. Hereditas(Beijing), doi: 10.16288/j.yczz.26-068.

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Membrane-anchored protein FvOPY2 regulates chitin and fumonisin biosynthesis and maize ear rot pathogenicity in Fusarium verticillioides

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