Abstract: Bovine leukocyte adhesion deficiency ( BLAD ) is autosomal recessive disease. The pathogeny of BLAD is genic mutation of CD18-integrins on the leukocyte. In order to know the carrier and occurrence of bovine leukocyte adhesion deficiency (BLAD) among cows age from one to six years old in China, 1 000 cows were investigated by means of amplifying a CD18 gene fragment via reverse transcriptase-PCR followed by restriction digestion with TaqⅠ. Results showed that 19 cows were BLAD carriers, indicating that the BLAD carrier rate was 1.9 percent. In adition, one cow was found to have BLAD.

Key words: analysis, flanking , rice, sequence, genetic , mutation, inserted , T-DNA