Abstract: A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence. The Chinese Muntjac’s mitochondrial genome, consisting of 16354 base pairs which encode genes for 13 proteins, 2 rRNAs, and 22 tRNAs, is similar to those mammals in both order and orientation.The sequence of rRNA gene ,some of the protein-coding regions and tRNAs are highly homologous in mammals. Differences existing in the length and sequence of the D-loop regions account for the variations in mammals mitochondrial genomes.

Key words: mitochondrial genome, Muntiacus reevesi, shot-gun sequencing, Sequence analysis