Changes of epigenetic modification and donor cell metabolic status can improve the developmental efficiency of canine-porcine interspecies somatic cell nuclear transfer embryos
Received date: 2025-04-17
Revised date: 2025-05-27
Online published: 2025-06-26
Supported by
Seed Industry Revitalisation Project of the Guangdong Provincial Department of Agriculture and Rural Affairs(2024-XPY-00-004)
To explore new ways to improve canine cloning efficiency, this study compared the effects of different concentrations and treatment times of glycolysis promoter PS48 and epigenetic modifiers (DNA methylase inhibitor RG108 and histone deacetylase inhibitor Scriptaid) on the developmental ability of canine-porcine interspecies somatic cell nuclear transfer (iSCNT) embryos. The results showed that (1) 5 μmol/L PS48 treatment on canine ear fibroblasts (cEFs) and canine adipose tissue-derived mesenchymal stem cells (cAd-MSCs) for 24 h significantly enhanced subsequent iSCNT embryo development. The cleavage rate, 4-cell stage rate and 8-cell stage rate of iSCNT embryos produced from PS48-treated cEFs were significantly higher than those of control iSCNT embryos (46.90±1.64% vs 13.30±1.61%, 32.30±1.55% vs 8.26±0.88%, and 10.62±1.68% vs 5.50±0.84%; P<0.05). The cleavage and 4-cell stage rates of iSCNT embryos generated from PS48-treated cAd-MSCs were significantly higher than those of control iSCNT embryos (49.51±3.00% vs 31.25±2.73%, 26.21±2.08% vs 15.18±1.58%; P<0.05). (2) Treatment of cEFs and cAd-MSCs with 20 μmol/L RG108 for 48 h had no significant effect on the developmental efficiency of iSCNT embryos. Treatment of cEFs and cAd-MSCs with 0 nmol/L, 400 nmol/L, 500 nmol/L and 600 nmol/L Scriptaid for 24 h had no significant effect on the developmental efficiency of iSCNT embryos. (3) Treatment of iSCNT embryos derived from two types of donor cells with 20 μmol/L RG108 significantly promoted their developmental competence (P<0.05). Treatment of iSCNT embryos derived from cEFs with 500 nmol/L Scriptaid for 16 h significantly increased their cleavage and 4-cell stage rates (23.08±2.94% vs 9.47±1.70%, 18.68±3.25% vs 6.32±1.07%; P<0.05). This study established some new methods that can significantly improve the developmental efficiency of canine-porcine iSCNT embryos, thereby contributing to the development and application of canine somatic cell cloning technology.
Yanxia Yan, Yuhan Zhang, Zhenfang Wu, Zicong Li . Changes of epigenetic modification and donor cell metabolic status can improve the developmental efficiency of canine-porcine interspecies somatic cell nuclear transfer embryos[J]. Hereditas(Beijing), 2025 , 47(12) : 1365 -1376 . DOI: 10.16288/j.yczz.25-109
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