遗传 ›› 2007, Vol. 29 ›› Issue (7): 898-904.doi: 10.1360/yc-007-0898

• 技术与方法 • 上一篇    

丝状真菌目标基因替换过程中的策略与方法

王教瑜1, 2; 杜新法1; 柴荣耀1; 孙国昌1; 林福呈2   

  1. 1. 浙江省农业科学院植物保护与微生物所, 杭州310021;
    2. 浙江大学农业与生物技术学院, 杭州310029

  • 收稿日期:2006-12-06 修回日期:2007-01-26 出版日期:2007-07-10 发布日期:2007-07-10
  • 通讯作者: 王教瑜

Strategies of targeted gene replacement in filamentous fungi

WANG Jiao-Yu1, 2; DU Xin-Fa1; CHAI Rong-Yao1; SUN Guo-Chang1; LIN Fu-Cheng2

  

  1. 1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;
    2. Colledage of Agriculture and Biotechnology, Zhejiang Universtiy, Hangzhou 310029, China
  • Received:2006-12-06 Revised:2007-01-26 Online:2007-07-10 Published:2007-07-10
  • Contact: WANG Jiao-Yu

摘要:

目标基因替换是基因功能分析的重要手段。随着基因组测序和转化技术的进展, 该技术在丝状真菌功能基因组学中的应用越来越广泛, 新的体系与方法不断建立和完善。文章在转化体系、打靶载体构建、突变体筛选等方面综述了丝状真菌目标基因替换过程中的策略与方法, 并对各方法的优缺点及发展趋势进行了评述。

关键词: 基因功能分析, 丝状真菌, 目标基因替换

Abstract:

Targeted gene replacement (TGR) is an important technique for gene function analysis. With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established. In this paper, strategies involved in TGR in filamentous fungi were reviewed, including transformation systems, targeting vector construction, mutant selection and so on. Compared with TGR, RNAi and other techniques of gene function analysis were introduced and reviewed as well.