LALBA基因SNP与内蒙古白绒山羊经济性状的关联

doi:10.3724/SP.J.1005.2008.00169

遗传 ›› 2008, Vol. 30 ›› Issue (2): 169-174.doi: 10.3724/SP.J.1005.2008.00169

LALBA基因SNP与内蒙古白绒山羊经济性状的关联

蓝贤勇¹; 陈宏^{1, 2}; 田智泉^{1, 3}; 刘少卿⁴; 张永斌⁴; 王昕¹; 房兴堂²

1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100;
2. 徐州师范大学细胞与分子生物学研究所, 徐州221166;
3. 扬州大学动物科学与技术学院, 扬州 225009;
4. 内蒙古鄂托克旗内蒙古阿尔巴白绒山羊种羊场 (三北羊场), 鄂尔多斯 016100

收稿日期:2007-08-07 修回日期:2007-10-28 出版日期:2008-02-10 发布日期:2008-02-10
通讯作者: 陈宏

Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat

LAN Xian-Yong¹; CHEN Hong ^1,2; TIAN Zhi-Quan ^1,3; LIU Shao-Qing⁴; ZHANG Yong-Bin ⁴; WANG Xin¹; FANG Xing-Tang ²

1. College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; 2. Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, Xuzhou 221116, China; 3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 4. Inner Mongolia White Cashmere Goat Breeding Farm, E’erduosi 016100, China

Received:2007-08-07 Revised:2007-10-28 Online:2008-02-10 Published:2008-02-10
Contact: CHEN Hong

摘要/Abstract

摘要：

利用PCR-SSCP和DNA测序技术检测452份内蒙古白绒山羊α-乳白蛋白(LALBA)基因单核苷酸多态性(SNP), 并分析SNP与产绒量、绒厚、绒长和体重性状的关联。结果表明, 仅P2引物位点存在SSCP多态, 其外显子3区域存在1个突变位点: M63868:g.1897T>C。内蒙古白绒山羊群体LALBA基因M63868:g.1897位点以TT型为主, T等位基因频率为0.983, 且处于Hardy-Weinberg平衡状态(P>0.05)。方差分析表明, LALBA基因M63868:g.1897位点多态仅与产绒量存在显著相关(P=0.017); 1897位点TC基因型个体产绒量比TT基因型个体多产绒142.68 g, 高26.21%, 且差异显著(P<0.05)。因此, TC基因型可作为山羊产绒性状标记辅助选择的有效DNA标记。

关键词: 经济性状, 关联, 单核苷酸多态性(SNP), α-乳白蛋白 (LALBA) 基因, 山羊

Abstract:

PCR-SSCP and DNA sequencing methods were conducted to detect single nucleotide polymorphism of alpha-lactalbumin (LALBA) gene in 452 Inner Mongolian white cashmere goats (IMWC). Correlations between SNP of goat LALBA gene and economic traits, e.g., cashmere yield, cashmere thickness, length and weight, were analyzed. The SSCP in P2 primer locus, which was caused by the point mutation M63868:g.1897T>C in the exon 3 of LALBA gene was detected. At this locus, the genotype TT and allele T were predominant in the IMWC population, which agreed with Hardy-Weinberg equilibrium. Moreover, there was a significant correlation between polymorphism of goat M63868:g.1897 locus and cashmere yield of IMWC (P=0.017). The individuals with genotype TC had more cashmere yield than those with geontype TT. Hence, genotype TC of LALBA gene can be used as a molecular marker for breeding superior cashmere yield in goat marker-assisted selection.

蓝贤勇，陈宏，田智泉，刘少卿，张永斌，王昕，房兴堂. LALBA基因SNP与内蒙古白绒山羊经济性状的关联[J]. 遗传, 2008, 30(2): 169-174.

LAN Xian-Yong, CHEN Hong, TIAN Zhi-Quan, LIU Shao-Qing, ZHANG Yong-Bin, WANG Xin, FANG Xing-Tang . Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat[J]. HEREDITAS, 2008, 30(2): 169-174.

[1]	宋绍征, 何正义, 成勇, 于宝利, 张婷, 李丹. TALENs介导MSTN基因突变山羊的制备及性能分析[J]. 遗传, 2022, 44(6): 531-542.
[2]	周子文, 王雪, 丁向东. 基于高密度SNP标记估计群体间遗传关联[J]. 遗传, 2021, 43(4): 340-349.
[3]	李以格, 张丹丹. 后GWAS时代结直肠癌致病SNP功能机制的研究进展[J]. 遗传, 2021, 43(3): 203-214.
[4]	孙一丹, 田子钊, 周伟, 李沫, 怀聪, 贺林, 秦胜营. 中国人群肝功能检测指标全基因组关联分析研究[J]. 遗传, 2021, 43(3): 249-260.
[5]	单婷玉, 施雯, 王翌婷, 曹孜怡, 汪保华, 方辉. 玉米盐胁迫相关性状全基因组关联分析及候选基因预测[J]. 遗传, 2021, 43(12): 1159-1169.
[6]	钱国清. 慢性阻塞性肺疾病全基因组关联研究进展[J]. 遗传, 2020, 42(9): 832-846.
[7]	周晨希, 李沫, 怀聪, 贺林, 秦胜营. 中国人群中抗结核药物引发肝损伤的易感基因标记研究[J]. 遗传, 2020, 42(4): 374-379.
[8]	宋绍征, 于康英, 张婷, 陆睿, 潘生强, 周鸣鸣, 成勇. tPA/gGH双基因转染山羊乳腺上皮细胞表达分析[J]. 遗传, 2020, 42(4): 380-387.
[9]	梅志超, 位竹君, 于佳慧, 冀凤丹, 解莉楠. 多组学关联分析揭示表观等位基因在拟南芥环境适应性进化中的作用及机制[J]. 遗传, 2020, 42(3): 321-331.
[10]	王冰源, 牟玉莲, 李奎, 刘志国. 农业动物干细胞研究进展[J]. 遗传, 2020, 42(11): 1073-1080.
[11]	梁文权,侯豫,赵存友. 精神分裂症相关单核苷酸多态性调控microRNA功能研究进展[J]. 遗传, 2019, 41(8): 677-685.
[12]	文超良, 孙从佼, 杨宁. 从遗传力到肠菌力：概念及研究进展[J]. 遗传, 2019, 41(11): 1023-1040.
[13]	王凤红,张磊,李晓凯,范一星,乔贤,龚高,严晓春,张令天,王志英,王瑞军,刘志红,王志新,何利兵,张燕军,李金泉,赵艳红,苏蕊. 山羊基因组研究进展[J]. 遗传, 2019, 41(10): 928-938.
[14]	彭哲也,唐紫珺,谢民主. 机器学习方法在基因交互作用探测中的研究进展[J]. 遗传, 2018, 40(3): 218-226.
[15]	林欣琪,梁融,张俊国,皮路程,陈思东,刘丽,郜艳晖. 稀有变异遗传关联性研究中常用负担检验方法比较[J]. 遗传, 2018, 40(2): 162-169.

LALBA基因SNP与内蒙古白绒山羊经济性状的关联

Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics