遗传 ›› 2008, Vol. 30 ›› Issue (3): 329-332.doi: 10.3724/SP.J.1005.2008.00329

• 研究报告 • 上一篇    下一篇

郏县红牛DGAT2基因多态性与生长性状的相关性分析

毛海霞1, 3; 陈宏1, 2; 陈付英1; 张春雷1; 王新庄4; 王居强4   

  1. 1. 西北农林科技大学动物科技学院 陕西省农业分子生物学重点实验室, 杨凌 712100;
    2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116;
    3. 山西师范大学生命科学院动科系, 临汾 041000;
    4. 河南省肉牛工程技术研究中心, 郑州 450003

  • 收稿日期:2007-08-16 修回日期:2007-11-15 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 陈宏

Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian red cattle

MAO Hai-Xia1,3; CHEN Hong1,2; CHEN Fu-Ying1; ZHANG Chun-Lei1; WANG Xin-Zhuang4; WANG Ju-Qiang4

  

  1. 1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; 2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 3. College of Life Science, Shanxi Normal University, Linfen 041000, China;
    4. Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003, China
  • Received:2007-08-16 Revised:2007-11-15 Online:2008-03-10 Published:2008-03-10
  • Contact: CHEN Hong

摘要:

利用PCR-RFLP技术对随机抽取的92头郏县红牛的DGAT2基因进行多态性分析。结果表明, DGAT2基因第6内含子的PCR扩增产物被限制性酶TaqⅠ酶切消化后表现多态性。经电泳检测后, 显出AA、AB两种不同基因型。该基因座处于Hardy-Weinberg平衡状态, 通过对郏县红牛DGAT2基因多态性与体重及体尺指标的方差分析显示:郏县红牛DGAT2基因不同基因型对郏县红牛体重和体长有显著影响(P<0.05), 而对其他体尺指标在统计学上无显著影响(P>0.05), 且初步认为AB基因型对选择有正向效应。

关键词: DGAT2, 生长性能, PCR-RFLP, 郏县红牛

Abstract:

The polymorphisms of DGAT2 gene in 92 Jiaxian red cattle were analyzed using PCR-RFLP method. Results showed that the PCR products digested with TaqI demonstrated polymorphisms, and showed two kinds of genotype: AA and AB. This polymorphic locus of DGAT2 gene was at Hardy-Weinberg equilibrium (P>0.05). The results of general linear model analysis showed that polymorphisms of DGAT2 gene had significant association (P<0.05) with body weight and body length, while had no significant association with others growth traits (P>0.05). Therefore, genotype AB may have positive effect on body weight.