遗传 ›› 2008, Vol. 30 ›› Issue (10): 1272-1278.doi: 10.3724/SP.J.1005.2008.01272

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单核苷酸多态性在林木中的研究进展

褚延广; 苏晓华

  

  1. 中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091

  • 收稿日期:2008-03-11 修回日期:2008-05-19 出版日期:2008-10-10 发布日期:2008-10-10
  • 通讯作者: 苏晓华

Research progress of single nucleotide polymorphisms in forest trees

CHU Yan-Guang;SU Xiao-Hua   

  1. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of For-estry (CAF), Beijing 100091, China
  • Received:2008-03-11 Revised:2008-05-19 Online:2008-10-10 Published:2008-10-10
  • Contact: SU Xiao-Hua

摘要:

摘要: 单核苷酸多态性(Single nucleotide polymorphisms, SNPs)是许多生物体最丰富的遗传变异形式。林木是重要的植物类群和陆地植物生态系统的重要组成部分, SNP作为新的分子标记已应用于松、杨、黄杉、桉和云杉等属的多个树种的遗传育种学研究, 获得了包括核苷酸多样性、连锁不平衡及群体结构等相关的遗传信息, 这些研究主要建立在对候选基因序列进行测序分析的基础上。基于SNP的关联遗传学分析或连锁不平衡(Linkage disequilibrium, LD)作图, 已成为研究林木复杂数量性状的理想工具, 对桉树和火炬松的关联遗传学研究发现, 多个基因内的SNP位点与不同的木材性状相关联。利用SNP标记对林木遗传参数的估算从不同程度上揭示了林木群体进化规律及其生态学意义。SNP标记在林木中应用的不断深入, 必将极大地推动林木遗传育种学研究的发展。

关键词: 关联遗传学, 连锁不平衡, 核苷酸多样性, 林木, 单核苷酸多态性

Abstract:

Abstract: Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variation in many organisms. Forest is an important plant group and key component of the terrestrial plant ecosystem. As a new marker system, SNP has been applied in genetic and breeding studies in some tree species of genera Pinus, Populus, Pseudotsuga, Eucalyptus, and Picea, and the related genetic information, such as nucleotide diversity, linkage disequilibrium (LD) and population struc-ture has been generated. These studies are mainly based on sequencing and analysis of candidate genes. SNP-based associa-tion genetics analysis or LD mapping has become a useful tool for dissection of complex traits in forest trees. Association studies in Eucalyptus spp. and Pinus taeda L. found that some SNP sites in various genes were associated with distinct wood property traits. Meanwhile, estimation of genetic parameters has revealed the evolutionary and ecological significance at different extents in several tree species. Intensive applications of SNP approach are expected to greatly accelerate the development of research on forest genetics and breeding.