关键词: SNPs, PCR-SSCP, 秦川牛, IGF2基因

Abstract:

PCR-SSCP technology was used to analyze the correlation of polymorphisms IGF2 (Insulin-like growth factor ) gene with carcass and meat quality traits in 186 Qinchuan cattle at the age of 24-month. C→T mutation in 120 and A→G mutation in 279 of IGF2 gene. Statistical analysis indicated that Qinchuan cattle with genotype BB and DD had significant differences in slaughter weight, carcass weight, carcass length, carcass chest depth and eye muscle area (P<0.05). The difference in thickness of back fat was significant(P<0.01). Significant differences in marbling, tenderness, pH24 of meat quality(P<0.05), but the differences in carcass depth and water holding capacity were not significant (P>0.05). Genotype AA, DD were predominant genotypes and A, D were predominant alleles. The population containing B, D allele had more excellent carcass and meat quality than others, especially in the capacity of fat accumulate