大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析

doi:10.3724/SP.J.1005.2009.00180

遗传 ›› 2009, Vol. 31 ›› Issue (2): 180-185.doi: 10.3724/SP.J.1005.2009.00180

大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析

任洪林¹,张文惠²,潘风光³,卢士英⁴,柳增善⁴

1. 人兽共患病教育部重点实验室,吉林大学人兽共患病研究所, 长春 130062;
2. 吉林大学军需科技学院, 长春 130062;
3. 福建省亚热带植物研究所, 厦门 361006

收稿日期:2008-05-06 修回日期:2008-09-04 出版日期:2009-02-10 发布日期:2009-02-10
通讯作者: 任洪林

Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus

REN Hong-Lin1, ZHANG Wen-Hui3, PAN Feng-Guang2, LU Shi-Ying1, LIU Zeng-Shan1，ZHANG Jun-Hui1

1. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China;
2. College of Light Industry and Economic, Jilin University, Changchun 130062, China;
3. Fujian Institute of Subtropical Botany, Xiamen 361006, China

Received:2008-05-06 Revised:2008-09-04 Online:2009-02-10 Published:2009-02-10
Contact: Hong-Lin REN

摘要/Abstract

摘要： 大腹园蛛大壶状腺表达拖丝蛋白新基因的克隆, 为进一步研究蛛丝蛋白基因以及人工表达蛛丝蛋白提供参考依据。文章利用“通用方法”即反转录—置换法构建大腹园蛛(Araneus ventricosus)大壶状腺(Major ampullate gland) cDNA文库, 并筛选出具有典型重复结构的大腹园蛛大壶状腺丝蛋白-1部分cDNA序列AvMaSp1 (GenBank登录号: AY177203)。该部分序列大小为1 408 bp, 编码区为1 288 bp, 编码氨基酸429个, 预测分子量为34.07 kDa, 典型的重复结构为 (GA)nAm(GA)N, 与十字园蛛(Araneus diadematus)丝蛋白基因ADF-1 (GenBank登录号: ADU47853)同源关系最近, 一致性为75.0%。

关键词: 蜘蛛丝, 拖丝, 蛛丝蛋白, 大壶状腺, 大腹园蛛

Abstract: In this paper, we successfully constructed the cDNA library of major ampullate gland of Araneus ventricousus using pUC18 vector and cloned the partial cDNA (AvMaSp1, GenBank accession number AY177203) encoding spider ma-jor ampullate gland spidroin-1 by means of picking colony randomly (Bird gun). The partial cDNA sequence of AvMaSp1 was 1 408 bp and its region in encoding was 1 288 bp. The protein deduced from AvMaSp1 contained 429 amino acid resi-dues and molecular weight was 34.07 kDa. The repetitive motif of this cDNA sequence was (GA)nAm(GA)N and the identity with A. diadematus fibroin-1 mRNA (ADF-1, GenBank accession number ADU47853) was 75.0%.

任洪林，张文惠，潘风光，卢士英，柳增善. 大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析[J]. 遗传, 2009, 31(2): 180-185.

REN Hong-Lin, ZHANG Wen-Hui, PAN Feng-Guang, LU Shi-Ying, LIU Zeng-Shan, ZHANG Jun-Hui. Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus[J]. HEREDITAS, 2009, 31(2): 180-185.

大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析

Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus

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