遗传 ›› 2009, Vol. 31 ›› Issue (5): 500-500―507.doi: 10.3724/SP.J.1005.2009.00500

• 研究报告 • 上一篇    下一篇

绵羊微卫星BMS2508FecB基因的多态及连锁分析

李延璐1;储明星2;陈宏权1;方丽2;狄冉2;马月辉2;李奎2   

  1. 1.安徽农业大学动物科技学院, 合肥 230036;
    2.中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与利用重点开放实验室, 北京 100193
  • 收稿日期:2008-12-15 修回日期:2009-03-15 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 储明星

Polymorphic and linkage analysis of microsatellite BMS2508 and FecB gene in sheep

LI Yan-Lu1;CHU Ming-Xing2;CHEN Hong-Quan1;FANG Li2;DI Ran2;MA Yue-Hui2;LI Kui2   

  1. 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2008-12-15 Revised:2009-03-15 Online:2009-05-10 Published:2009-05-10
  • Contact: MingXing Chu

摘要: 文章分析与绵羊高繁殖力主效基因FecB紧密连锁的微卫星座位BMS2508在高繁殖力绵羊品种(小尾寒羊)和低繁殖力绵羊品种(特克塞尔、多赛特和中国美利奴)中的遗传多态性, 同时探讨该微卫星座位与小尾寒羊FecB基因的连锁不平衡关系。高繁殖力品种小尾寒羊在骨形态发生蛋白受体IB(Bone morphogenetic protein receptor IB, BMPR-IB)基因编码序列第746位碱基处发生了与Booroola Merino绵羊相同的FecB突变(A746G), 而在低繁殖力的特克塞尔、多赛特和中国美利奴绵羊中没有检测到该突变; 小尾寒羊BB、B+、++的基因型频率分别为0.485、0.398和0.117。微卫星座位BMS2508在4个绵羊品种的438个个体中共检测到8个等位基因和15种基因型, 最小等位基因为94 bp, 最大等位基因为116 bp; 小尾寒羊(n = 307)、特克塞尔(n = 45)、多赛特(n = 46)、中国美利奴(n = 40)和BB型(n = 149)、B+型(n = 122)、++型(n = 36)小尾寒羊群体中优势等位基因分别是100 bp、94 bp、94 bp、112 bp、100 bp、100 bp、112 bp, 其频率分别为0.453、0.544、0.802、0.475、0.483、0.439、0.389。连锁不平衡分析显示小尾寒羊FecB基因B等位基因与BMS2508微卫星座位100 bp等位基因之间存在一定的连锁不平衡(D′=0.408), 而+等位基因与BMS2508微卫星座位110 bp和114b p等位基因均存在一定的连锁不平衡(D′=0.513)。

关键词: 绵羊, 多羔性, 连锁不平衡, FecB基因, BMS2508

Abstract: Genetic polymorphisms of microsatellite locus BMS2508, which was closely linked to the ovine fecundity gene FecB, were detected in prolific (Small Tail Han sheep) and non-prolific breeds of sheep (Texel, Dorset and Chinese Merino). The linkage disequilibrium between microsatellite locus BMS2508 and FecB gene of Small Tail Han sheep was also ana-lyzed. There was the same mutation (A746G) of BMPR-IB gene in Small Tail Han sheep as that of FecB in Booroola Me-rino ewes, but the FecB mutation was absent in Texel, Dorset and Chinese Merino sheep. The genotype frequencies of BB, B+ and ++ were 0.485, 0.398 and 0.117 in Small Tail Han sheep, respectively. There were eight alleles varied from 94 bp to 116 bp and 15 genotypes detected at BMS2508 locus in four sheep breeds totally 438 individual. The preponderant allele was 100 bp, 94 bp, 94 bp, 112 bp, 100 bp, 100 bp, 112 bp, and the frequency was 0.453, 0.544, 0.802, 0.475, 0.483, 0.439, 0.389 in Small Tail Han (n=307), Texel (n=45), Dorset (n=46), Chinese Merino (n=40), and BB group (n=149), B+ group (n=122), ++ group (n=36) from Small Tail Han, respectively. In Small Tail Han sheep, linkage analysis indicated that there was certain linkage disequilibrium between 100 bp allele of microsatellite BMS2508 and B allele of FecB gene (D′ =0.408), and certain linkage disequilibrium between 110 bp and 114 bp alleles of microsatellite BMS2508 and + allele of FecB gene (D′=0.513).