猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系

doi:10.3724/SP.J.1005.2010.01153

遗传 ›› 2010, Vol. 32 ›› Issue (11): 1153-1158.doi: 10.3724/SP.J.1005.2010.01153

猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系

钱源, 曾勇庆, 杜金芳, 崔景香, 李华, 陈其美, 宋一萍, 陈伟

山东农业大学动物科技学院, 泰安 271018

收稿日期:2010-01-14 修回日期:2010-04-10 出版日期:2010-11-20 发布日期:2010-10-25
通讯作者: 曾勇庆 E-mail:yqzeng@sdau.edu.cn
基金资助:
国家转基因重大专项(编号：2008ZX08006-002), 国家高技术研究发展计划“863”重点项目(编号：2008AA101008), 山东省农业良种工程重大项目(编号：2009LZ012-03)和山东省自然科学基金项目(编号：Y2008D32)资助

CDS cloning and relationship between intramuscular fat content and mRNA expression of PID1 gene in pig

QIAN Yuan, ZENG Yong-Qing, DU Jin-Fang, CUI Jing-Xiang, LI Hua, CHEN Qi-Mei, SONG Yi-Ping, CHEN Wei

College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China

Received:2010-01-14 Revised:2010-04-10 Online:2010-11-20 Published:2010-10-25
Contact: ZENG Yong-Qing E-mail:yqzeng@sdau.edu.cn

摘要/Abstract

摘要： 为了探索PID1(Phosphotyrosine interaction domain containing 1)基因的表达与脂肪沉积的关系, 文章利用兼并引物进行RT-PCR从猪脂肪和肌肉组织中克隆PID1基因CDS(Coding region)区全序列, 并采用荧光定量PCR方法对大白猪、鲁莱黑猪、莱芜猪3个猪品种的肝脏、脂肪和肌肉组织PID1基因mRNA表达进行了相对定量分析。结果表明: 经克隆、测序, 得到了猪PID1基因654 bp全编码区序列, 通过Blast比对, 与人、大鼠、牛有93.88%、66.94%、88.07%的同源性。PID1基因在同一个品种猪中mRNA表达水平总体表现为: 肝脏>脂肪>肌肉。在不同品种3种组织中PID1基因mRNA表达水平总体表现为: 莱芜猪>鲁莱黑猪>大白猪, 其中肝脏中差异显著(P<0.05), 但是在脂肪和肌肉组织中莱芜猪与鲁莱黑猪差异不显著(P>0.05)。对于高肌内脂肪(LWH)、中等肌内脂肪(LWI)和低肌内脂肪(LWL)沉积的3组莱芜猪, PID1基因在肝脏组织中的表达水平是LWH显著高于LWL(P<0.05), 在肌肉组织中则是LWH显著高于LWI和LWL(P<0.05)。PID1基因在莱芜猪品种内3个组织中mRNA表达量与IMF含量相关均不显著, 而在品种间3个组织中mRNA表达量与IMF含量呈显著正相关(P<0.05)。结果提示: PID1的表达可能与脂肪沉积性状存在一定的关系。

关键词: 猪, PID1基因, 组织表达, 肌内脂肪

Abstract: To explore the relationship between the expression of PID1 gene and fat deposition, we cloned CDS of PID1 from porcine fat and muscle tissues by RT-PCR using degenerate primers, and investigated expression of this gene in various tissues (i.e., liver, backfat, and muscle tissues) of different breeds (i.e., Yorkshire, Laiwu, and Lulai Black) by real-time fluorescence quantitative PCR. The results showed that 654 bp CDS of porcine PID1 was obtained by sequencing and was 93.88%, 66.94% and 88.07% identical to those of the human, rat, and Bos taurus, respectively. The expression of PID1 mRNA in various tissues and breeds, on the whole, tended to be liver > fat > muscle and Laiwu > Lulai Black > Yorkshire, respectively. For different breeds, PID1 mRNA abundance in liver had significant difference (P<0.05), but had no signifi-cant differences in fat and muscle tissues between Laiwu and Lulai Black (P>0.05). For the three groups of Laiwu pigs with high (LWH), intermediate (LWI), and low IMF content (LWL), PID1 mRNA level was higher in liver tissue of LWH than that of LWL significantly (P<0.05), and was higher in muscle tissue of LWH than that of LWI and LWL significantly (P<0.05). PID1 mRNA abundance was not correlated with IMF in these three tissues of Laiwu breed, but it was positively correlated with IMF in the tissues of these three breeds (P<0.05). These results implied that the expression of PID1 may be related to fat deposition.

Key words: pig, PID1 gene, tissue expression, intramuscular fat

钱源，曾勇庆，杜金芳，崔景香，李华，陈其美，宋一萍，陈伟. 猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系[J]. 遗传, 2010, 32(11): 1153-1158.

JIAN Yuan, CENG Yong-Qiang, DU Jin-Fang, CUI Jing-Xiang, LI Hua, CHEN Ji-Mei, SONG Yi-Ping, CHEN Wei. CDS cloning and relationship between intramuscular fat content and mRNA expression of PID1 gene in pig[J]. HEREDITAS, 2010, 32(11): 1153-1158.

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猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系

CDS cloning and relationship between intramuscular fat content and mRNA expression of PID1 gene in pig

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