内源ABA信号水平动态调控的分子机制

doi:10.3724/SP.J.1005.2012.00296

遗传 ›› 2012, Vol. 34 ›› Issue (3): 296-306.doi: 10.3724/SP.J.1005.2012.00296

内源ABA信号水平动态调控的分子机制

魏开发¹, 陈娟¹, 陈艳峰¹, 吴凌娟¹, 贾文锁²

1. 漳州师范学院生物科学与技术系, 福建漳州 363000 2. 中国农业大学农学与生物技术学院, 北京 100193

收稿日期:2011-11-05 修回日期:2011-12-26 出版日期:2012-03-20 发布日期:2012-03-25
通讯作者: 魏开发 E-mail:kaifa-wei@163.com
基金资助:
福建省自然科学基金(编号: B0810040), 福建省教育厅A类科技项目(编号: JA08154)和2010及2011年度福建省大学生创新性实验计划项目(编号: 125, No.25)

Molecular mechanism for dynamic regulation of endogenous ABA signal level

WEI Kai-Fa¹, CHEN Juan¹, CHEN Yan-Feng¹, WU Ling-Juan¹, JIA Wen-Suo²

1. Department of Biological Sciences and Biotechnology, Zhangzhou Normal University, Zhangzhou 363000, China 2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

Received:2011-11-05 Revised:2011-12-26 Online:2012-03-20 Published:2012-03-25
Contact: WEI Kai-Fa E-mail:kaifa-wei@163.com

摘要/Abstract

摘要： 从逆境信号感知、ABA合成的触发到ABA水平的动态调控, 是细胞内重要的逆境信号传导途径, 相对于应答ABA的下游信号事件, 该领域研究滞后。研究显示, 根系中ZEP、限速酶NCED、AtRGS1等合成酶基因及ABA2基因响应胁迫反应上调ABA信号水平。而7′-, 8′-, 9′-hydroxylase和糖基转移酶基因受逆境诱导激活, 负调节ABA的积累。同时, 提高的内源ABA信号水平能激活合成酶基因和代谢酶基因的表达。此外, 基因表达和源库动力学分析显示, 叶片ABA动态库的维持依赖根源ABA的持续供应。值得一提的是, miRNA与ABA信号起源及动态水平维持有关。进一步的代谢动力学分析揭示, ABA信号水平受合成酶基因和代谢酶基因表达的协同控制, 多因素共同参与内源ABA信号水平的动态调控。

关键词: ABA信号水平, 合成调节, 反馈调节, 代谢调节, 转运调节, miRNA途径, 协同控制

Abstract: The process from stress signal perception and the trigger of ABA biosynthesis to dynamic regulation of ABA level is an important stress signaling pathway in cells. Compared to the downstream events in ABA signal transduction, the researches in this field are relatively lagged. Expression of synthase genes, such as ZEP in roots and rate-limiting enzyme genes NCED, AtRGS1 and ABA2, can be activated in response to stresses. However, the expression of genes encoding degradative enzymes, including 7′-, 8′-, 9′-hydroxylase and glucosyltransferase, negatively regulates ABA accumulation. Meanwhile, the expressions of the synthases, such as ZEP and NCED3, are induced by increasing en-dogenous ABA contents. Additionally, the analyses of gene expression and source-sink dynamics indicates that sustained supply from root-sourced ABA is required for the maintenance of leaf ABA dynamic pool. It is notable that miRNAs should be involved in ABA signal origin and ABA level dynamic adjustment. Further dynamic analysis of ABA metabolism re-vealed that endogenous ABA signal levels are synergistically controlled by the expressions of synthases and degradative enzymes.

Key words: degradative regulation, feedback regulation, transportation regulation, miRNA pathway, synergistic control, ABA signal levels, biosynthetic regulation

魏开发，陈娟，陈艳峰，吴凌娟，贾文锁. 内源ABA信号水平动态调控的分子机制[J]. 遗传, 2012, 34(3): 296-306.

WEI Kai-Fa, CHEN Juan, CHEN Yan-Feng, WU Ling-Juan, JIA Wen-Suo. Molecular mechanism for dynamic regulation of endogenous ABA signal level[J]. HEREDITAS, 2012, 34(3): 296-306.

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内源ABA信号水平动态调控的分子机制

Molecular mechanism for dynamic regulation of endogenous ABA signal level

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