遗传 ›› 2013, Vol. 35 ›› Issue (3): 307-314.doi: 10.3724/SP.J.1005.2013.00307
刘芳,卢长明
收稿日期:2012-06-04
修回日期:2012-07-25
出版日期:2013-03-20
发布日期:2013-03-25
通讯作者:
卢长明
E-mail:cmlu@oilcrops.cn
基金资助:油菜KCS基因家族底物特异性及异位表达研究项目(编号：31100190) 资助
LIU Fang, LU Chang-Ming
Received:2012-06-04
Revised:2012-07-25
Online:2013-03-20
Published:2013-03-25
摘要： 植物非特异脂质转运蛋白(nsLTP)是一类含量丰富的小分子碱性蛋白, 能够在体外与多种疏水分子可逆地结合。目前已从多种植物中分离到9种类型的nsLTP基因。所有nsLTP蛋白质都具有8个半胱氨酸残基模体的保守结构, 它们的三维结构内部有一个具有脂质结合位点的疏水腔。根据基因结构、表达、调控和体外活性等研究, nsLTP被认为可能与蜡质合成与运输、抗逆、抗病以及生殖发育等重要生理过程有关。文章全面介绍nsLTP基因及其蛋白质研究的最新进展, 内容包括基本特征、分类、基因表达、基因克隆与功能研究等, 最后对今后的研究方向进行了讨论和展望。
中图分类号:
刘芳，卢长明. 植物非特异脂质转运蛋白研究现状与展望[J]. 遗传, 2013, 35(3): 307-314.
LIU Fang, LU Chang-Ming. An overview of non-specific lipid transfer protein in plant[J]. HEREDITAS, 2013, 35(3): 307-314.
| [1] Douliez JP, Michon T, Elmorjani K, Marion D. Mini review: structure, biological and technological functions of lipid transfer proteins and indolines, the major lipid bind-ing proteins from cereal kernels. J Cereal Sci, 2000, 32(1): 1-20.[2] Hamilton JA. Fatty acid interactions with proteins: what X-ray crystal and NMR solution structures tell us. Prog Lipid Res, 2004, 43(3): 177-199.[3] DeBono A, Yeats TH, Rose JKC, Bird D, Jetter R, Kunst L, Samuels L. Arabidopsis LTPG is a glycosylphosphati-dylinositol-anchored lipid transfer protein required for export of lipids to the plant surface. Plant Cell, 2009, 21(4): 1230-1238.[4] Lee SB, Go YS, Bae HJ, Park JH, Cho SH, Cho HJ, Lee DS, Park OK, Hwang I, Suh MC. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglob-ules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola. Plant Physiol, 2009, 150(1): 42-54.[5] Pasquato N, Berni R, Folli C, Folloni S, Cianci M, Pantano S, Helliwell JR, Zanotti G. Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens. J Mol Biol, 2006, 356(3): 684-694.[6] Hoh F, Pons JL, Gautier MF, de Lamotte F, Dumas C. Structure of a liganded type 2 non-specific lipid-transfer protein from wheat and the molecular basis of lipid binding. Acta Crystallogr D, 2005, 61(4): 397-406.[7] Castro MS, Gerhardt IR, Orrù S, Pucci P, Bloch Jr C. Purification and characterization of a small (7.3 kDa) putative lipid transfer protein from maize seeds. J Chro-matogr B, 2003, 794(1): 109-114.[8] Lauga B, Charbonnel-Campaa L, Combes D. Characterization of MZm3-3, a Zea mays tapetum-specific tran-script. Plant Sci, 2000, 157(1): 65-75.[9] Boutrot F, Guirao A, Alary R, Joudrier P, Gautier MF. Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim Biophys Acta Gene Struct Exp, 2005, 1730(2): 114-125.[10] Boutrot F, Chantret N, Gautier MF. Genome-wide analysis of the rice and Arabidopsis non-specific lipid transfer protein (nsLtp) gene families and identification of wheat nsLtp genes by EST data mining. BMC Genomics, 2008, 9(1): 86-105.[11] Edstam MM, Viitanen L, Salminen TA, Edqvist J. Evolutionary history of the non-specific lipid transfer proteins. Mol Plant, 2011, 4(6): 947-964.[12] Liu KD, Jiang H, Moore SL, Watkins CB, Jahn MM. Isolation and characterization of a lipid transfer protein ex-pressed in ripening fruit of Capsicum chinense. Planta, 2006, 223(4): 672-683.[13] Feng JX, Ji SJ, Shi YH, Wei G, Zhu YX. Analysis of five differentially expressed gene families in fast elongating cotton fiber. Acta Biochim Biophys Sin, 2004, 36(1): 51-57.[14] Arondel V, Vergnolle C, Cantrel C and Kader JC. Lipid transfer proteins are encoded by a small multigene family in Arabidopsis thaliana. Plant Sci, 2000, 157(1): 1-12.[15] Boutrot F, Meynard D, Guiderdoni E, Joudrier P, Gautier MF. The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice. Planta, 2007, 225(4): 843-862.[16] Guiderdoni E, Cordero MJ, Vignols F, Garcia-Garrido JM, Lescot M, Tharreau D, Meynard D, Ferrière N, Notteghem J, Delseny M. Inducibility by pathogen attack and devel-opmental regulation of the rice Ltp1 gene. Plant Mol Biol, 2002, 49(6): 683-699.[17] Buhtz A, Kolasa A, Arlt K, Walz C, Kehr J. Xylem sap protein composition is conserved among different plant species. Planta, 2004, 219(4): 610-618.[18] Cameron KD, Teece MA, Smart LB. Increased accumula-tion of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiol, 2006, 140(1): 176-183.[19] Choi AM, Lee SB, Cho SH, Hwang I, Hur CG, Suh MC. Isolation and characterization of multiple abundant lipid transfer protein isoforms in developing sesame (Sesamum indicum L.) seeds. Plant Physiol Biochem, 2008, 46(2): 127-139.[20] Panikashvili D, Shi JX, Bocobza S, Franke RB, Schreiber L, Aharoni A. The Arabidopsis DSO/ABCG11 transporter affects cutin metabolism in reproductive organs and suberin in roots. Mol Plant, 2010, 3(3): 563-575.[21] Zhang D, Wengier D, Shuai B, Gui CP, Muschietti J, McCormick S, Tang WH. The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth. Plant Physiol, 2008, 148(3): 1368-1379.[22] Zhang DS, Liang WQ, Yin CS, Zong J, Gu FW, Zhang DB. OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiol, 2010, 154(1): 149-162.[23] 刘志勇, 叶雪凌, 李承彧, 冯辉. 大白菜核雄性不育相关基因BrLTP1的克隆及特征分析. 园艺学报, 2011, 38(2): 343-352.[24] Edqvist J, Farbos I. Characterization of germination-specific lipid transfer proteins from Euphorbia lagascae. Planta, 2002, 215(1): 41-50.[25] Eklund DM, Edqvist J. Localization of nonspecific lipid transfer proteins correlate with programmed cell death responses during endosperm degradation in Euphorbia la-gascae seedlings. Plant Physiol, 2003, 132(3): 1249-12591.[26] Maldonado AM, Doerner P, Dixon RA, Lamb CJ, Cam-eron RK. A putative lipid transfer protein involved in sys-temic resistance signalling in Arabidopsis. Nature, 2002, 419(6905): 399-403.[27] Jang CS, Johnson JW, Seo YW. Differential expression of TaLTP3 and TaCOMT1 induced by Hessian fly larval in-festation in a wheat line possessing H21 resistance gene. Plant Sci, 2005, 168(5): 1319-1326.[28] Chae K, Kieslich C, Morikis D, Kim SC, Lord EM. A gain-of-function mutation of Arabidopsis lipid transfer protein 5 disturbs pollen tube tip growth and fertilization. Plant Cell, 2009, 21(12): 3902-3914.[29] Chae K, Gonong BJ, Kim SC, Kieslich CA, Morikis D, Balasubramanian S, Lord EM. A multifaceted study of stigma/style cysteine-rich adhesion (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction. J Exp Bot, 2010, 61(15): 4277-4290.[30] Jang CS, Kim DS, Bu SY, Kim JB, Lee SS, Kim JY, Johnson JW, Seo YW. Isolation and characterization of lipid transfer protein (LTP) genes from a wheat-rye trans-location line. Plant Cell Rep, 2002, 20(10): 961-966.[31] Gaudet DA, Laroche A, Frick M, Huel R, Puchalski B. Cold induced expression of plant defensin and lipid trans-fer protein transcripts in winter wheat. Physiol Plantarum, 2003, 117(2): 195-205.[32] Yubero-Serrano E-M, Moyano E, Medina-Escobar N, Muñoz-Blanco J, Caballero J-L. Identification of a straw-berry gene encoding a non-specific lipid transfer protein that responds to ABA, wounding and cold stress. J Exp Bot, 2003, 54(389): 1865-1877.[33] Wu GH, Robertson AJ, Liu XJ, Zheng P, Wilen RW, Nesbitt NT, Gusta LV. A lipid transfer protein gene BG-14 is differentially regulated by abiotic stress, ABA, aniso-mycin, and sphingosine in bromegrass (Bromus inermis). J Plant Physiol, 2004, 161(4): 449-458.[34] Hwang EW, Kim KA, Park SC, Jeong MJ, Byun MO, Kwon HB. Expression profiles of hot pepper (Capsicum annuum) genes under cold stress conditions. J Biosci, 2005, 30(5): 657-667.[35] Liu KH, Lin TY. Cloning and characterization of two novel lipid transfer protein I genes in Vigna radiate. DNA Seq, 2003, 14(6): 420-426.[36] Jung HW, Kim KD, Hwang BK. Identification of pathogen-responsive regions in the promoter of a pepper lipid transfer protein gene (CALTPI) and the enhanced resistance of the CALTPI transgenic Arabidopsis against pathogen and environmental stresses. Planta, 2005, 221(1): 361-373.[37] Gonorazky AG, Regente MC, de la Canal L. Stress induction and antimicrobial properties of a lipid transfer protein in germinating sunflower seeds. J Plant Physiol, 2005, 162(6): 618-624.[38] Oshino T, Abiko M, Saito R, Ichiishi E, Endo M, Kawagishi-Kobayashi M, Higashitani A. Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature injury in barley plants. Mol Genet Genomics, 2007, 278(1): 31-42.[39] Gorjanovi? S, Su?njevi? D, Beljanski M, Hranisavljevi? J. Barley lipid-transfer protein as heavy metal scavenger. Environ Chem Lett, 2004, 2(3): 113-116.[40] Jang CS, Lee HJ, Chang SJ, Seo YW. Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.). Plant Sci, 2004, 167(5): 995-1001.[41] 郑明. 植物非特异性脂转移蛋白基因的多抗性功能初步研究[学位论文]. 长春: 吉林大学, 2009.[42] Federico ML, Kaeppler HF, Skadsen RW. The complex developmental expression of a novel stress-responsive barley Ltp gene is determined by a shortened promoter sequence. Plant Mol Biol, 2005, 57(1): 35-51.[43] Maghuly F, Borroto-Fernandez EG, Khan MA. Expression of calmodulin and lipid transfer protein genes in Prunus incisa × serrula under different stress conditions. Tree Physiol, 2009, 29(3): 437-444.[44] Wang C, Yang CP, Gao CQ, Wang YC. Cloning and ex-pression analysis of 14 lipid transfer protein genes from Tamarix hispida responding to different abiotic stresses. Tree Physiol, 29 (12): 1607-1619.[45] Jung HW, Kim W, Hwang BK. Three pathogen-inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environ-mental stresses. Plant Cell Environ, 2003, 26(6): 915-928.[46] Park CJ, Shin R, Park JM, Lee GJ, You JS, Paek KH. In-duction of pepper cDNA encoding a lipid transfer protein during the resistance response to tobacco mosaic virus. Plant Mol Biol, 2002, 48(3): 243-254.[47] Kirubakaran SI, Begum SM, Ulaganathan K, Sakthivel N. Characterization of a new antifungal lipid transfer protein from wheat. Plant Physiol Biochem, 2008, 46(10): 918-927.[48] Sterk P, Booij H, Schellekens GA, van Kammen A, de Vries SC. Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell, 1991, 3(9): 907-921.[49] Kunst L, Samuels AL. Biosynthesis and secretion of plant cuticular wax. Prog Lipid Res, 2003, 42(1): 51-80.[50] Hincha D K. Cryoprotectin: a plant lipid-transfer protein homologue that stabilizes membranes during freezing. Phil Trans R Soc Lond B, 2002, 357(1423): 909-916.[51] Riederer M, Schreiber L. Protecting against water loss: analysis of the barrier properties of plant cuticles. J Exp Bot, 2001, 52(363): 2023-2032.[52] 李健. 转脂质转运蛋白基因山新杨抗逆性分析[学位论文]. 哈尔滨: 东北林业大学, 2010.[53] 李诚斌. 水稻OsLTP1基因的克隆、表达分析及功能鉴定[学位论文]. 南宁: 广西大学, 2004.[54] Patkar RN, Chattoo BB. Transgenic indica rice expressing nsLTP like protein shows enhanced resistance to both fungal and bacterial pathogens. Mol Breed, 2006, 17(2): 159-171.[55] 唐征, 杨凯, 冯永庆, 曹庆芹, 沈元月, 秦岭. 板栗脂质转运蛋白基因的克隆及表达. 林业科学, 2010, 46(4): 43-48.[56] Gomés E, Sagot E, Gaillard C, Laquitaine L, Poinssot B, Sanejouand YH, Delrop S, Coutos-Thévenot P. Nonspecific lipid transfer protein genes expression in grape (Vitis sp.) cells in response to fungal elicitor treatments. Mol Plant Microbe Interact, 2003, 16(5): 456-464.[57] Roy-Barman S, Sautter C, Chattoo BB. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses. Transgenic Res, 2006, 15(4): 435-446.[58] de Oliveira Carvalho A, Gomes VM. Role of plant lipid transfer proteins in plant cell physiology: a concise review. Peptides, 2007, 28(5): 1144-1153.[59] Sarowar S, Kim YJ, Kim KD, Hwang BK, Ok SH, Shin JS. Overexpression of lipid transfer protein (LTP) genes en-hances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. Plant Cell Rep, 2009, 28(3): 419-4271.[60] Buhot N, Douliez J-P, Jacquemard A, Marion D, Tran V, Maume BF, Milat ML, Ponchet M, Mikès V, Kader JC, Blein JP. A lipid transfer protein binds to a receptor involved in the control of plant defence responses. FEBS Lett, 2001, 509(1): 27-30.[61] Blein JP, Coutos-Thevenot P, Marion D, Ponchet M. From elicitins to lipid-transfer proteins: a new insight in cell signalling involved in plant defence mechanisms. Trends Plant Sci, 2002, 7(7): 293-296.[62] Buhot N, Gomès E, Milat ML, Ponchet M, Marion D, Lequeu J, Delrot S, Coutos-Thévenot P, Blein JP. Modulation of the biological activity of a tobacco LTP1 by lipid complexation. Mol Biol Cell, 2004, 15(11): 5047-5052.[63] Lascombe MB, Bakan B, Buhot N, Marion D, Blein JP, Larue V, Lamb C, Prange T. The structure of “defective in induced resistance” protein of Arabidopsis thaliana, DIR1, reveals a new type of lipid transfer protein. Prot Sci, 2008, 17(9): 1522-1530.[64] Jung HW, Tschaplinski TJ, Wang L, Glazebrook J, Greenberg JT. Priming in systemic plant immunity. Science, 2009, 324(5923): 89-91.[65] Ariizumi T, Amagai M, Shibata D, Hatakeyama K, Watanabe M, Toriyama K. Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana. Plant Cell Rep, 2002, 21(1): 90-96.[66] Imin N, Kerim T, Weinman JJ, Rolfe BG. Low tempera-ture treatment at the young microspore stage induces protein changes in rice anthers. Mol Cell Proteomics, 2006, 5(2): 274-292.[67] Park SY, Jauh GY, Mollet JC, Nothnagel EA, Walling LL, Lord EM. A lipid transfer-like protein is necessary for lily pollen tube adhesion to an in vitro stylar matrix. Plant Cell, 2000, 12(1): 151-164.[68] Chae K, Zhang KL, Zhang L, Morikis D, Kim ST, Mollet JC, de la Rosa N, Tan K, Lord EM. Two SCA (stigma/ style cysteine-rich adhesin) isoforms show structural differences that correlate with their levels of in vitro pollen tube adhesion activity. J Biol Chem, 2007, 282(46): 33845-33858.[69] Nieuwland J, Feron R, Huisman BAH, Fasolino A, Hilbers CW, Derksen J, Mariani C. Lipid transfer proteins enhance cell wall extension in tobacco. Plant Cell, 2005, 17(7): 2009-2019.[70] Sieber P, Schorderet M, Ryser U, Buchala A, Kolattukudy P, Metraux JP, Nawrath C. Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions. Plant Cell, 2000, 12(5): 721-737.[71] Tomassen MMM, Barrett DM, Valk HCPM, Woltering EJ. Isolation and characterization of a tomato non-specific lipid transfer protein involved in polygalacturonase-mediated pectin degradation. J Exp Bot, 2007, 58(5): 1151-1160.[72] Pii Y, Astegno A, Peroni E, Zaccardelli M, Pandolfini T, Crimi M. The Medicago truncatula N5 gene encoding a root-specific lipid transfer protein is required for the symbiotic interaction with Sinorhizobium meliloti. Mol Plant Microbe In, 2009, 22(12): 1577-1587.[73] Salcedo G, Sanchez-Monge R, Diaz-Perales A, Garcia-Casado G, Barber D. Plant non-specific lipid transfer proteins as food and pollen allergens. Clin Exp Al-lergy, 2004, 34(9): 1336-1341.[74] Lauer I, Dueringer N, Pokoj S, Rehm S, Zoccatelli G, Reese G, Miguel-Moncin MS, Cistero-Bahima A, Enrique E, Lidholm J, Vieths S, Scheurer S. The non-specific lipid transfer protein, Ara h 9, is an important allergen in peanut. Clin Exp Allergy, 2009, 39(9): 1427-1437.[75] Zoccatelli G, Pokoj S, Foetisch K, Bartra J, Valero A, Del Mar San Miguel-Moncin M, Vieths S, Scheurer S. Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3). Mol Immunol, 2010, 47(7-8): 1561- 1568.[76] Jang CS, Yim WC, Moon JC, Hung JH, Lee TG, Lim SD, Cho SH, Lee KK, Kim W, Seo YW, Lee BM. Evolution of non-specific lipid transfer protein (nsLTP) genes in the Poaceae family: their duplication and diversity. Mol Genet Genomics, 2008, 279(5): 481-497.[77] Wang HW, Hwang SG, Karuppanapandian T, Liu AH, Kim W, Jang CS. Insight into the molecular evolution of non-specific lipid transfer proteins via comparative analysis between rice and sorghum. DNA Res, 2012, 19(2): 179-194.[78] Wang HW, Kwon HJ, Yim WC, Lim SD, Moon JC, Lee BM, Seo YW, Kim W, Jang CS. Expressional diversity of wheat nsLTP genes: evidence of subfunctionalization via cis-regulatory divergence. Genetica, 2010, 138(8): 843-852. |
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