猪microRNA组学研究进展

doi:10.3724/SP.J.1005.2014.0974

遗传 ›› 2014, Vol. 36 ›› Issue (10): 974-984.doi: 10.3724/SP.J.1005.2014.0974

猪microRNA组学研究进展

冉茂良^1,2,陈斌^1,2,尹杰^3,4,杨岸奇^1,2,李智^1,2,蒋明^1,2

1. 湖南农业大学动物科学技术学院,长沙 410128;
2. 畜禽遗传改良湖南省重点实验室,长沙 410128;
3. 中国科学院亚热带农业生态研究所,长沙 410125;
4. 中国科学院研究生院,北京 100049

收稿日期:2014-05-04 出版日期:2014-10-20 发布日期:2014-10-20
通讯作者: 陈斌,教授,博士生导师,研究方向：猪的遗传育种。E-mail: chenbin7586@126.com E-mail:ranmaoliang0903@126.com
作者简介:冉茂良,博士研究生,专业方向：猪的遗传育种。E-mail: ranmaoliang0903@126.com
基金资助:
国家现代农业产业技术体系建设专项资金(CARS-36)资助

Advances in porcine miRNAome

Maoliang Ran^{1, 2}, Bin Chen^{1, 2}, Jie Yin^{3, 4}, Anqi Yang^{1, 2}, Zhi Li^{1, 2}, Ming Jiang^{1, 2}

1. College of Animal Science & Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Changsha 410128, China;
3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
4. University of the Chinese Academy of Sciences, Beijing 100049, China

Received:2014-05-04 Online:2014-10-20 Published:2014-10-20

摘要/Abstract

摘要： MicroRNA(miRNA)是一类长约22 nt的非编码小RNA,广泛存在于各种生物中,调节生物体生长、发育和凋亡等过程。研究表明,miRNA在猪肌肉、脂肪、生殖系统以及免疫系统等的发育过程中发挥着重要的调控作用。此外,高通量的新一代测序技术在猪miRNA的挖掘和差异表达研究中发挥着巨大的作用。文章综述了高通量的新一代测序技术在挖掘猪miRNA中的应用以及一些miRNA在猪脂肪代谢、肌肉发育、卵母细胞成熟和B、T淋巴细胞发育中的调控作用,旨在为猪miRNA的研究提供参考,为利用miRNA调控和改善猪肉品质、生长性能、繁殖性能以及免疫机能提供理论基础和研究思路。

关键词: 猪, microRNA, 脂肪代谢, 肌肉发育, 卵母细胞成熟, 免疫

Abstract: MicroRNA (miRNA), a class of non-coding RNA (about 22 nt), widely exists in different organisms and plays an important role in growth, development, and apoptosis. Recently, many studies have shown that miRNAs serve as a regulatory mechanism in mediating the development of pig muscle, fat, reproductive and immunity traits. The next-generation of high-throughput sequencing technology plays a critical role on finding new miRNAs and identifying their different expressions in pig. In this review, we summarize the application of the next-generation of high-throughput sequencing technology in the study of pig miRNAs as well as the regulatory roles of several important miRNAs in fat metabolism, muscle development, oocyte maturation and development of B cells and T cells. This review will provide insight into the research on pig miRNAs, and some ideas on how to improve pork quality, growth, reproductive and immunity performance.

Key words: porcine, miRNA, fat metabolism, muscle development, oocyte maturation, immunity

冉茂良,陈斌,尹杰,杨岸奇,李智,蒋明. 猪microRNA组学研究进展[J]. 遗传, 2014, 36(10): 974-984.

Maoliang Ran, Bin Chen, Jie Yin, Anqi Yang, Zhi Li, Ming Jiang. Advances in porcine miRNAome[J]. HEREDITAS(Beijing), 2014, 36(10): 974-984.

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猪microRNA组学研究进展

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