水稻抗逆相关基因的分子进化分析

doi:10.3724/SP.J.1005.2014.1027

遗传 ›› 2014, Vol. 36 ›› Issue (10): 1027-1035.doi: 10.3724/SP.J.1005.2014.1027

水稻抗逆相关基因的分子进化分析

宋晓军^1,2,谢凯斌³,张艳萍¹,金萍²

1. 青岛农业大学生命科学学院,山东省高校植物生物技术重点实验室,青岛 266109;
2. 南京师范大学生命科学学院,比较基因组学与生物信息学实验室,南京 210023;
3. 南京师范大学教师教育学院,南京 210023

收稿日期:2014-03-05 出版日期:2014-10-20 发布日期:2014-10-20
通讯作者: 金萍,博士,讲师,专业方向：功能基因组学与进化生物学。E-mail: jinping8312@gmail.com E-mail:sxjwyj@163.com
作者简介:宋晓军,博士,讲师,专业方向：功能基因组学与进化生物学。E-mail: sxjwyj@163.com
基金资助:
江苏省青年基金项目(编号：BK20130902)和江苏省普通高校研究生科研创新计划项目(编号：CXZZ13_0411)资助

The molecular evolution of rice stress-related genes

Xiaojun Song^{1, 2}, Kaibin Xie³, Yanping Zhang¹, Ping Jin²

1. Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China;
2. Laboratory for Comparative Genomics and Bioinformatics, College of Life Science, Nanjing Normal University, Nanjing 210023, China;
3. College of Teacher Education, Nanjing Normal University, Nanjing 210023, China

Received:2014-03-05 Online:2014-10-20 Published:2014-10-20

摘要/Abstract

摘要： 植物在进化过程中为了适应外界环境,已经具有一套完整的抵抗外界特殊环境的调控系统。但是,关于水稻抗逆相关基因的分子进化方面的研究还未见报道。文章通过Plant Tolerance Gene Database数据库,获得22个水稻抗逆相关基因。利用比较基因组学和生物信息学方法对水稻抗逆相关基因的进化动态进行研究,结果表明水稻抗逆相关基因在低等植物中比较保守;随着植物的不断进化和生存环境的改变,其基因数量也随之增加。具有相似抗性功能的基因往往具有相似的基因结构和基序(motif)结构。文章还发现4个保守motif 的存在：HRDXK、DXXSXG、LLPR和GXGXXG(X代表任意氨基酸)。在GSK1、RAN2抗逆基因中发现了3个特有的motif结构：GSK1特有的P-rich motif,RAN2特有的G-rich motif和E-rich motif。推测这些保守的motif结构与基因的抗逆功能密切相关。进化速率分析结果表明,尽管植物抗逆性相关基因在进化过程中受到较强的纯化选择作用,但是仍然有50%的抗逆性相关基因存在正选择位点。这些正选择位点的存在有可能为基因适应外界环境变化提供了重要的物质基础。

关键词: 水稻, 抗逆相关基因, 进化, 选择压力

Abstract: In the processes of evolution, plants have formed a perfect regulation system to tolerate adverse environmental conditions. However, there has not been any report about the molecular evolution of rice stress-related genes. We derived a family of 22 stress-related genes in rice from Plant Stress Gene Database, and analyzed it by bioinformatics and comparative genome method. The results showed that these genes are relatively conservative in low organisms, and their copy numbers increase along with the environmental changes and the evolution. We also found four conserved sequence motifs and three other specific motifs. We propose that these motifs are closely associated with the function of rice stress-related genes. The analysis of selection pressure showed that about 50% rice stress-related genes have positive selection sites, although they were subject to a strong purifying selection. Positive selection sites might be very significant for plants to adapt to environmental changes.

Key words: rice, stress-related genes, evolution, selective pressure

宋晓军,谢凯斌,张艳萍,金萍. 水稻抗逆相关基因的分子进化分析[J]. 遗传, 2014, 36(10): 1027-1035.

Xiaojun Song, Kaibin Xie, Yanping Zhang, Ping Jin. The molecular evolution of rice stress-related genes[J]. HEREDITAS(Beijing), 2014, 36(10): 1027-1035.

参考文献

[1] MM, Pereira JS, Maroco J, Rodrigues ML, Ricardo CPP, Osório ML, Carvalho I, Faria T, Pinheiro C. How plants cope with water stress in the field? Photosynthesis and growth . Ann Bot , 2002, 89(7): 907-916.
[2] V, Doskočilová A, Komis G, Šamaj J. Crosstalk between secondary messengers, hormones and MAPK modules during abiotic stress signalling in plants. Biotechnol Adv , 2014, 32(1): 2-11.
[3] B, Nguyen HT. Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Curr Opin Plant Biol , 2006, 9(2): 189-195.
[4] KB, Foley RC, Oñate-Sánchez L. Transcription factors in plant defense and stress responses. Curr Opin Plant Biol , 2002, 5(5): 430-436.
[5] HT, Guo P, Xia XL, Yin WL. PdERECTA, a leucine-rich repeat receptor-like kinase of poplar, confers enhanced water use efficiency in Arabidopsis. Planta , 2011, 234(2): 229-241.
[6] SK, Kim BG, Kwon TR, Jeong MJ, Park SR, Lee JW, Byun MO, Kwon HB, Matthews BF, Hong CB, Park SC. Overexpression of the mitogen-activated protein kinase gene OsMAPK33 enhances sensitivity to salt stress in rice ( Oryza sativa L . ). J Biosci , 2011, 36(1): 139-151.
[7] R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol , 2008, 59: 651-681.
[8] N. Abscisic acid and abiotic stress signaling. Plant Signal Behav , 2007, 2(3): 135-138.
[9] P, Hey SJ, Halford NG. The sucrose non-fermen-ting-1-related (SnRK) family of protein kinases: potential for manipulation to improve stress tolerance and increase yield. J Exp Bot , 2011, 62(3): 883-893.
[10] AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R. Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol , 2010, 17(5): 596-601.
[11] M, Lotze MT, Holton N. Receptor-mediated signalling in plants: molecular patterns and programmes. J Exp Bot , 2009, 60(13): 3645-3654.
[12] Y, Li X, Tan H, Liu M, Zhao X, Wang J. Mol-ecular characterization of RsMPK2 , a C1 subgroup mitogen-activated protein kinase in the desert plant Reaumuria soongorica . Plant Physiol Biochem , 2010, 48(10-11): 836- 844.
[13] MCS, Petersen M, Mundy J. Mitogen-activated protein kinase signaling in plants. Annu Rev Plant Biol , 2010, 61: 621-649.
[14] G, Agarwal P, Grant M, Kumar A. MAPK machinery in plants: recognition and response to different stresses through multiple signal transduction pathways. Plant Signal Behav , 2010, 5(11): 1370-1378.
[15] R, Barkla BJ, Bohnert HJ, Pantoja O. Novel regulation of aquaporins during osmotic stress. Plant Physiol , 2004, 135(4): 2318-2329.
[16] M, Bertrand C, Matton DP. Characterization of a fertilization-induced and developmentally regulated plasma-membrane aquaporin expressed in reproductive tissues, in the wild potato Solanum chacoense Bitt. Planta , 2002, 215(3): 485-493.
[17] X, Tousch D, Ferrare K, Legrand E, Dupuis JM, Casse-Delbart F, Lamaze T. Two TIP-like genes encoding aquaporins are expressed in sunflower guard cells. Plant J , 1997, 12(5): 1103-1111.
[18] GM, Komatsu S. Proteomic analysis of rice leaf sheath during drought stress. J Proteome Res , 2006, 5(2): 396-403.
[19] ZQ, Targolli J, Huang XQ, Wu R. Wheat LEA genes, PMA80 and PMA1959, enhance dehydration tolerance of transgenic rice ( Oryza sativa L.). Mol Breed , 2002, 10(1-2): 71-82.
[20] K, Walton LJ, Tunnacliffe A. LEA proteins prevent protein aggregation due to water stress. Biochem J , 2005, 388(Pt 1): 151-157.
[21] PF, Shen QX, Ho TD. Structure and promoter analysis of an ABA-and stress-regulated barley gene, HVA1 . Plant Mol Biol , 1994, 26(2): 617-630.
[22] M, Webb R, Balsamo R, Close TJ, Yu XM, Griffith M. Purification, immunolocalization, cryoprotective, and antifreeze activity of PCA60: a dehydrin from peach ( Prunus per

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

水稻抗逆相关基因的分子进化分析

The molecular evolution of rice stress-related genes

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	卞中, 曹东平, 庄文姝, 张舒玮, 刘巧泉, 张林. 水稻分子设计育种启示：传统与现代相结合[J]. 遗传, 2023, 45(9): 718-740.
[2]	刘向东, 吴锦文, 陆紫君, Muhammad Qasim Shahid. 同源四倍体水稻：低育性机理、改良与育种展望[J]. 遗传, 2023, 45(9): 781-792.
[3]	郝小花, 胡爽, 赵丹, 田连福, 谢子靖, 吴莎, 胡文俐, 雷晗, 李东屏. OsGA3ox通过合成不同活性GA调控水稻育性及株高[J]. 遗传, 2023, 45(9): 845-855.
[4]	郑镇武, 赵宏源, 梁晓娅, 王一珺, 王驰航, 巩高洋, 黄金燕, 张桂权, 王少奎, 刘祖培. 水稻qGL3.4调控籽粒大小与株型[J]. 遗传, 2023, 45(9): 835-844.
[5]	陈明江, 刘贵富, 肖叶青, 余泓, 李家洋. 中科发早粳1号分子设计育种[J]. 遗传, 2023, 45(9): 829-834.
[6]	刘永强, 李威威, 刘昕禹, 储成才. 水稻分蘖氮响应调控机理研究进展[J]. 遗传, 2023, 45(5): 367-378.
[7]	姜明亮, 郎红, 李晓楠, 祖野, 赵靖, 彭沈凌, 刘振, 战宗祥, 朴钟云. 植物孤基因研究进展[J]. 遗传, 2022, 44(8): 682-694.
[8]	李姗, 黄允智, 刘学英, 傅向东. 作物氮肥利用效率遗传改良研究进展[J]. 遗传, 2021, 43(7): 629-641.
[9]	张昌泉, 冯琳皓, 顾铭洪, 刘巧泉. 江苏省水稻品质性状遗传和重要基因克隆研究进展[J]. 遗传, 2021, 43(5): 425-441.
[10]	巴恒星, 胡鹏飞, 李春义. 鹿科动物基因组学研究进展[J]. 遗传, 2021, 43(4): 308-322.
[11]	吕孟冈, 刘艾嘉, 李庆伟, 苏鹏. RHR转录因子家族起源、功能以及进化机制的研究进展[J]. 遗传, 2021, 43(3): 215-225.
[12]	代航, 李延, 刘树春, 林磊, 吴娟燕, 张志杰, 彭崎春, 李楠, 张向前. 类伸展蛋白OsPEX1对水稻花粉育性的影响[J]. 遗传, 2021, 43(3): 271-279.
[13]	章誉兴, 吴宏, 于黎. 哺乳动物毛色调控机制及其适应性进化研究进展[J]. 遗传, 2021, 43(2): 118-133.
[14]	罗鑫, 宿兵. 三维基因组分析点亮人类大脑进化之谜[J]. 遗传, 2021, 43(2): 105-107.
[15]	闫凌月, 张豪健, 郑雨晴, 丛韫起, 刘次桃, 樊帆, 郑铖, 袁贵龙, 潘根, 袁定阳, 段美娟. 转录因子OsMADS25提高水稻对低温的耐受性[J]. 遗传, 2021, 43(11): 1078-1087.