遗传 ›› 1997, Vol. 19 ›› Issue (5): 1-4.

• 论文 •    下一篇

玉米cyclinⅢ基因的染色体原位杂交物理定位

任南; 宋运淳; 毕学知; 刘立华 REN Nan;SONG Yun-Chun;BI Xue-Zhi;LIU Li-Hua   

  1. 武汉大学生命科学院, 武汉 430072
  • 收稿日期:1900-01-01 出版日期:1997-10-10 发布日期:1997-10-10

The Physical Location of the Maize cyclin III Gene by in situ Hypbridization

  • Received:1900-01-01 Online:1997-10-10 Published:1997-10-10

摘要: 本文首次报道了玉米低拷贝基因 cyclinⅢ(B-类)生物素标记的染色体原位杂交定位结果。供试探针为该基因的cDNA克隆,其长度仅为1.6kb。结果表明, 探针的信号分布在第6染色体短臂和第9染色体长臂,与着丝粒的百分距离分别为70.05±3.31和86.86±1.64,检出率分别为8.29%和6.83%。文中对基因的物理位置与功能间的关系等作了讨论。
Abstract:A biotin-labelled in situ hybridization technique was used to physically map a low copy gene cyclinIII in maize.The cDNA clone was 1.7kb in size.The probe was hybridized onto the short arm of chromosome 6 and the long arm of chromosome 9.The percent distances from centromere to detection site were 70.05±3.31 and 86.86±1.64 respetively.The detection rates of in situ hybridization were 8.29 and 6.83 respectively,The relationship between the position and function of the genes is discussed in this paper.

关键词: 染色体原位杂交, Maize, 生物素标记
Key words,
cyclinⅢ基因, 玉米, 表型差异, P68 RNA解旋酶, 细胞系

Key words: P68 RNA helicases, Phenotypic differences, Cell lines