遗传 ›› 1999, Vol. 21 ›› Issue (2): 21-642.

• 论文 • 上一篇    下一篇

黄颡鱼染色体的C-带、Ag-NORs、荧光带和复制带显带的研究

耿德贵;张大生;程伟;邹寿昌;陈刚 GENG De-Gui;ZHANG Da-Sheng;CHENG Wei;ZOU Shou-Chang;CHEN Gang      

  1. 徐州师范大学生物系,徐州221009 Department of Biology,Xuzhou Normal University of Jiangsu Province,Xuzhou221009
  • 收稿日期:1900-01-01 出版日期:1999-04-10 发布日期:1999-04-10

Chromosome Studies inPelteobagrus fulvidracoby C-banding,Ag-NORs, Fluorochrome Staining and Replication Banding

  • Received:1900-01-01 Online:1999-04-10 Published:1999-04-10

摘要: 采用Giemas染色、C-带、Ag-NORs、荧光染色和复制带显带的技术对黄颡鱼染色体进行了研究。结果表明, 黄颡鱼只有部分的染色体呈现阳性C-带, 可分为三类, 其中NORs区是染色最深、染色面积最大的区域, 为深染居间C-带。其Ag-NORs位于m5q末端。CMA3染色显示 NORs区呈现出明亮的荧光。中复制染色体上着丝粒区、端粒区和居间区浅染。发现核仁缢痕、深染居间C-带、Ag-NORs、CMA3明亮区和中复制带浅染NORs区位置基本一致, C-带阳性区和中复制带浅染区具有对应性。
Abstract Metaphase chromosomes of Pelteobagrus fulvidraco were analyzed by means of Giemsa staining, C-banding, Ag-NORs, fluorochrome staining and replication banding. Only parts of chromosomes showed C-band positive, the C-band heterochromatin was located in three regions. The NORs-bearing chromosomes had the largest and strongest C-bands. The long arms of chromosome pair 5 were the regions showing positive labeling with Ag-staining. Fluorochrome CMA3positively stained the NORs. In mid-period, negative replication bands were located in the centromere, the terminal and interstitial regions of the chromosomes. The distributions of secondary ?constri-ctions, positive C-bands, Ag-NORs, positive fluorescence bands and negative replication bands of mid-period were coincident.
  
  

关键词: 复制带 Key words Pelteobagrus fulvidraco, 荧光染色, Ag-NORs, C-带, 黄颡鱼