遗传 ›› 2001, Vol. 23 ›› Issue (5): 480-481.

• 论文 • 上一篇    下一篇

一种新的cDNA 末端快速扩增获取全长cDNA的方法

邱为民;张思仲;武辉;张戈;肖翠英 QIU Wei-min;ZHIANG Si-zhong;WU Hui;ZHANG Ge;XIAO Cui-ying   

  1. 四川大学华西医院医学遗传研究室,都 610041 Department of Medical Genetics,First University Hospital,West China Medical Center,Sichuan University,Chengdu 610041,P.R.China
  • 收稿日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

A Novel Method of Rapid Amplification of cDNA Ends for Obtaining the Full-Length cDNA

  • Received:1900-01-01 Online:2001-10-10 Published:2001-10-10

摘要: 为克隆精子发生相关基因的全长cDNA,根据mRNA差异显示获得的ESTs设计引物,利用一种新的cDNA末端快速扩增方法(SMARTRACE)扩增该EST的5′末端,并进行克隆测序,与mRNA差异显示获得ESTs拼接后,获得了三个新的全长cDNA.结果表明,SMARTRACE是一种简便、有效的克隆cDNA5′末端未知序列的技术.
Abstract:To clone the full-length cDNAs of genes related to spermatogenesis,ESTs obtained by mRNA differential display were used to design gene-specific primer.Then SMART RACE was performed to obtain the 5′ region of these ESTs.After cloning,sequencing and splicing with ESTs obtained by mRNA differential display,three full-length cDNAs were obtained.The results indicate that SMART RACE is a simple and an effective technique for cloning 5′-end unknown sequence of gene.

关键词: SMART RACE, spermatogenesis-related gene, 精子发生相关基因
Key words,
cDNA 末端快速扩增