遗传 ›› 2001, Vol. 23 ›› Issue (6): 535-306.

• 论文 • 上一篇    下一篇

大鼠部分肝切除前后热激处理对过氧化物酶基因表达的影响

徐存拴1;张为民1;王莹2;赵绪永1 XU Cun-shuan1;ZHANG Wei-min1;WANG Ying2;ZHAO Xu-yong1   

  1. 1.河南师范大学生命科学学院,河南,新乡,453002 2.暨南大学生命科学技术学院,广州,510632 1.College of Life Sciences,Henan Normal University,Xinxiang,Henan 453002,China; 2.Collgeg of Life Science and Technology,Jinan University,Guangzhou 510632,China
  • 收稿日期:1900-01-01 出版日期:2001-12-10 发布日期:2001-12-10

Expression Difference of the Peroxidase Genes in Partial Hepatectomy and Heat Shock

  • Received:1900-01-01 Online:2001-12-10 Published:2001-12-10

摘要: 用过氧化物酶原位复性电泳(SDS-POD-PAGE)技术分析了部分肝切除(partial hepatectomy,PH)、部分肝切除后再热激(partial hepatectomyfollowing heat shock,PH-HS)和先热激再部分肝切除(heat shock follow ing partial hepatectomy,HS-PH)后肝再生期间过氧化物酶(POD)基因表达差异,结果表明,PH中表达的POD基因种类(7个)>PH-HS(5个)>HS-PH(3个);三个模型的POD总活性为:HS-PH>PH>PH-HS.根据实验结果推测,POD在肝再生和肝损伤恢复中起一定作用。
Abstract:Expression difference of the peroxidase(POD)genes during liver regeneration after partial hepatectomy(PH),partial hepatectomy following heat shock(PH-HS)and heat shock following partial hepatectomy(HS-PH)was analyzed by POD renatured electrophoresis(SDS-POD-PAGE).The results showed that the expressed POD gene kinds in PH,PH-HS and HS-PH were 7,5 and 3 respectively,and that the total activities of POD in the three models were HS-PH>PH>PH-HS.The results suggested that POD play some roles during liver regeneration and in recovery after liver damage.

关键词: 诱导酶, 热激(HS), 部分肝切除(PH), 过氧化物酶(POD), 等位酶