摘要： 本文简要介绍简并PCR技术，包括什么是简并引物，如何设计简并引物，进行简并PCR的反应条件，应用简并PCR获得全长基因的方法和简并PCR技术的应用范围，并对简并PCR技术的局限性及其新进展进行讨论。在此基础上，简述基因的克隆策略以及简并PCR技术在基因克隆中的应用。简并PCR技术是寻找和发现“新”基因或蛋白质家族新成员的一种非常有用的工具。
Abstract:Degenerate PCR is introduced in this paper,including what is degenerate PCR,how to design degenerate primers,how to optimize degenerate PCR parameters,how to applying degenerate PCR to obtain full-length gene and which fields can apply degenerate PCR.The limits and recent advances of degenerate PCR are also discussed.Based on this introduction,strategies of gene cloning and applications of degenerate PCR in gene cloning are summarized in brief.Degenerate PCR is a very useful tool for searching and discovering new genes and new members of a protein family.