遗传 ›› 2004, Vol. 26 ›› Issue (6): 991-.

• 论文 • 上一篇    

T-DNA整合的研究进展

杨继芳;刘明;安利佳   

  1. 大连理工大学环境与生命学院,大连,116023
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-12-10 发布日期:2004-12-10

The Progress on T-DNA Integration Research

YANG Ji-Fang;LIU Ming;AN Li-Jia   

  1. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, 116023, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-12-10 Published:2004-12-10

摘要: 根癌农杆菌介导的基因转化过程中,T-DNA的整合是关系到外源基因能否稳定遗传的关键步骤。影响T-DNA整合的因素很多,包括毒性蛋白、寄主因子等等,本文对此加以综述,同时阐述了T-DNA在植物基因组中的整合及染色体水平上的分布及其整合模型。

关键词: 整合效率, 边界序列, 毒性蛋白, T-DNA整合

Abstract: The T-DNA integration is a critical step of the steady inheritance for foreign genes during the Agrobacterium tumefaciens-mediated transformation. There are many factors that influence the integration, including virulence proteins, host factors and so on.The article reviews the recent progress in these aspects, and also expatiates on the integration of T-DNA in host genome and distribution in the chromosomal level and the integration models.