遗传 ›› 2006, Vol. 28 ›› Issue (9): 1161-1166.

• 研究报告 • 上一篇    下一篇

粪便DNA突变检测在快速筛选大肠癌中的应用

吴文娟1; 周国华1,2   

  1. 1. 中国药科大学生命科学与技术学院, 南京 210009; 2. 华东医学生物技术研究所, 南京 210002

  • 收稿日期:2005-11-10 修回日期:2006-01-13 出版日期:2006-09-01 发布日期:2006-09-01
  • 通讯作者: 周国华

The Development of Rapid Screening of Colorectal Cancer by Mutation Detection in Fecal DNA

WU Wen-Juan1; ZHOU Guo-Hua1, 2

  

  1. 1. College of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China; 2. Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China

  • Received:2005-11-10 Revised:2006-01-13 Online:2006-09-01 Published:2006-09-01
  • Contact: ZHOU Guo-Hua

摘要: 大肠癌是发病率和死亡率都很高的常见疾病, 对高危人群进行大规模的普查筛选, 可以降低大肠癌的发生率和死亡率。粪便DNA突变检测是新近发展的可用作大肠癌普查的技术, 与常规结肠镜检测和大便隐血实验相比, 具有特异性高、灵敏度高和易于被患者接受等优点。文章阐述了粪便DNA突变检测的相关基因、肿瘤特异性DNA提取方法和检测方法等, 并对其在快速筛选大肠癌中的应用进行了展望。大肠癌是发病率和死亡率都很高的常见疾病, 对高危人群进行大规模的普查筛选, 可以降低大肠癌的发生率和死亡率。粪便DNA突变检测是新近发展的可用作大肠癌普查的技术, 与常规结肠镜检测和大便隐血实验相比, 具有特异性高、灵敏度高和易于被患者接受等优点。文章阐述了粪便DNA突变检测的相关基因、肿瘤特异性DNA提取方法和检测方法等, 并对其在快速筛选大肠癌中的应用进行了展望。

关键词: 大肠癌, 提取方法, 粪便DNA突变检测, 筛选

Abstract:

Colorectal cancer is one of the leading causes of death in the world. Screening high-risk populations for colorectal cancer can lower both the incidence of and deaths from the disease. Mutation detection in fecal DNA is a new approach of screening patients for colorectal cancer. Compared with colonoscopy and fecal occult-blood test, this approach has the advantages of high specificity, high sensitivity and general acceptability. This paper systematically reviewed genes associated with fecal DNA testing, methods for extracting tumor-specific DNA, and methods for detecting gene mutations. Moreover, the application of mutation detection using fecal DNA for rapid screening of colorectal cancer was briefly discussed.

中图分类号: