pRNA的结构、功能及其研究近况

遗传 ›› 2006, Vol. 28 ›› Issue (9): 1180-1184.

pRNA的结构、功能及其研究近况

周辉; 卢向阳; 田云; 黄成江

湖南农业大学生化与发酵工程实验室, 长沙 410128

收稿日期:2005-10-02 修回日期:2006-01-13 出版日期:2006-09-01 发布日期:2006-09-01
通讯作者: 周辉

The Structure, Function and Recent Reseach of pRNA

ZHOU Hui; LU Xiang-Yang; TIAN Yun; HUANG Cheng-Jiang

Laboratory of Biochemistry ＆ Fermentation Engineering, Hunan Agricultural University, Changsha 410128, China

Received:2005-10-02 Revised:2006-01-13 Online:2006-09-01 Published:2006-09-01
Contact: ZHOU Hui

摘要/Abstract

摘要：

在噬菌体phi29中, 基因组DNA的包装需要由病毒基因组编码的pRNA参与, 6个pRNA分子通过由pRNA分子间相互作用形成的六聚体来启动DNA转运马达, 这个过程由ATP提供能量。RNA纳米技术将pRNA与siRNA、核酶、反义RNA等分子稳定结合, pRNA作为一种载体把它们准确运输到癌细胞和病毒感染细胞的作用靶点, 从而发挥它们各自的功能。作为一种非编码RNA, 对pRNA的深入研究将有助于我们了解生命起源问题, 并有着广阔的应用前景。

关键词: pRNA, 六聚体, DNA转运, 纳米粒子

Abstract:

In the bacteriophage phi29, a virus-encoded RNA(pRNA) is required for the packaging of its genomic DNA. Six pRNAs form a hexamer through pRNA/pRNA interaction to drive the DNA translocation motor, and ATP as the energy resource. Chimeric pRNAs can steadily combine with siRNA, ribozymes and anti-sense RNA, and then be transferred into the cancer cells and viral-infected cells by RNA nanotechnology. The continued study of pRNA will help us to understand the origin of the life and explore its possible applications.

中图分类号:

周辉，卢向阳，田云，黄成江. pRNA的结构、功能及其研究近况[J]. 遗传, 2006, 28(9): 1180-1184.

ZHOU Hui, LU Xiang-Yang, TIAN Yun, HUANG Cheng-Jiang. The Structure, Function and Recent Reseach of pRNA[J]. HEREDITAS, 2006, 28(9): 1180-1184.