Ⅱ型糖尿病患者APOA5-1131T/C基因多态性与血脂代谢和胰岛素抵抗的关系研究

遗传 ›› 2007, Vol. 29 ›› Issue (5): 541-541―546.

Ⅱ型糖尿病患者APOA5-1131T/C基因多态性与血脂代谢和胰岛素抵抗的关系研究

翟光华¹; 闻平²; 郭兰芳¹; 陈璐¹

1. 江苏大学医学技术学院, 镇江212013;
2. 江苏省镇江市第一人民医院检验科, 镇江 212001

收稿日期:2006-07-22 修回日期:2006-01-10 出版日期:2007-05-08 发布日期:2007-05-08
通讯作者: 闻平

Association of apolipoprotein A5 gene -1131T/C polymorphism with lipid metabolism and insulin resistance in patients with type Ⅱ dia-betes mellitus

ZHAI Guang-Hua¹; WEN Ping²; GUO Lan-Fang¹; CHEN Lu¹

1. School of Medical Technology, Jiangsu University, Zhenjiang 212013, China;
2. The First Pepole’s Hospital of Zhenjiang Sity, Zhenjiang 212001, China

Received:2006-07-22 Revised:2006-01-10 Online:2007-05-08 Published:2007-05-08
Contact: WEN Ping

摘要/Abstract

摘要：

为了探讨载脂蛋白A5基因(APOA5)-1131T/C多态性在中国镇江地区的频率分布及其与血浆脂质代谢和Ⅱ型糖尿病患者胰岛素抵抗的关系, 采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)结合琼脂糖凝胶电泳技术检测152例健康人及71例Ⅱ型糖尿病患者APOA5 -1131T/C基因型及等位基因频率分布, 同时采用生化方法测定所有研究对象的血脂、血糖和胰岛素水平。结果显示: 糖尿病组APOA5 -1131C等位基因频率显著高于对照组(0.430 vs 0.296, P = 0.006)。CC纯合子患糖尿病的风险是TT纯合子的3.75倍(OR = 3.75, 95% CI: 1.57~8.92), 且经Logistic回归分析, 校正年龄、BMI和血浆HDL-c、LDL-c及ApoB水平等其他混杂因素影响后, 这种差异仍具有显著性意义(OR = 2.70, 95%CI: 1.24 ~ 5.86)。糖尿病组C等位基因携带者TG水平显著高于非C等位基因携带者(P < 0.01), TC水平和LDL-c水平亦明显升高(P < 0.05)。但是在两组中, 不同基因型患者胰岛素抵抗相关指标均无显示差异。提示APOA5-1131T/C单核苷酸多态性对人群血浆TG水平有影响, -1131C等位基因与血浆TG、TC和LDL-c水平增高有关, 但是与糖尿病患者胰岛素相关指标无关; APOA5 -1131C等位基因可能与人群糖尿病的发生相关联。

关键词: 单核苷酸多态性(SNP), 载脂蛋白A5基因(APOA5), 甘油三酯(TG), 胰岛素抵抗(IR)

Abstract:

The purpose of this study was to explore the frequency of apolipoprotein A5 (APOA5) -1131T/C polymorphism in Zhenjiang and its effects on lipid metabolism and insulin resistance in type Ⅱ diabetes mellitus(DM) patients. The genotypes of APOA5 -1131T/C polymorphism were determined in 152 healthy individuals and 71 type Ⅱ DM patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum levels of lipids, glucose and insulin in these subjects were also estimated by biochemical methods. The frequency of the APOA5-1131C allele in DM patients was significantly higher than that of the control group (0.430 vs 0.296, P = 0.006). When compared with the TT genotype, CC homozygotes had a significantly increased DM risk (OR=3.75, 95% CI: 1.57-8.92). In the DM group, the serum levels of triglyceride (TG) of C carriers (TC+CC) were significantly higher than those of non-C carriers (TT) (P<0.01), and serum levels of total cholesterol (TC) and low density lipoprotein cholesterol(LDL-c) in subjects with the CC genotype were also significantly higher than those with the TT genotype (P < 0.05). However, there was no significance in profiles of insulin resistance in various genotypes in both groups. The APOA5 single nucleotide polymorphism was associ-ated with serum TG level in the population. The -1131C allele contributed to the increase of serum levels of TG, TC and LDL-c and but had no effect on profiles of insulin resistance in DM patients. The APOA5 -1131C allele may be associated with increased susceptibility to type Ⅱ diabetes mellitus．

翟光华，闻平，郭兰芳，陈璐. Ⅱ型糖尿病患者APOA5-1131T/C基因多态性与血脂代谢和胰岛素抵抗的关系研究[J]. 遗传, 2007, 29(5): 541-541―546.

ZHAI Guang-Hua, WEN Ping, GUO Lan-Fang, CHEN Lu. Association of apolipoprotein A5 gene -1131T/C polymorphism with lipid metabolism and insulin resistance in patients with type Ⅱ dia-betes mellitus[J]. HEREDITAS, 2007, 29(5): 541-541―546.

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