关键词: 水稻, 锌指蛋白, 非生物胁迫, 表达分析, 基因克隆

Abstract:

A rice zinc-finger protein gene, RZF71, encoding the C2H2-type zinc-finger transcription factor was isolated from rice (Oryza sativa L. subs. Japonica) by RT-PCR approach. Gene RZF71 encodes a 25 kDa protein with 250 amino acids, which contains two typical C2H2 zinc finger domains. The expression profiling showed that RZF71 was constitutively expressed in roots, culms, leaves, and flowering spikes. The semi-quantitative RT-PCR assay showed RZF71 was strongly induced by high-salinity and 20% PEG6000 treatments, but not regulated by low temperature and ABA (abscisic acid) treatments. Tran-sient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. These results indicated that the RZF71 may play an important role in rice responses to salt and osmotic stresses as a transcription factor.