遗传 ›› 2008, Vol. 30 ›› Issue (3): 324-328.doi: 10.3724/SP.J.1005.2008.00324

• 研究报告 • 上一篇    下一篇

中国家驴的非洲起源研究

卢长吉1, 谢文美1, 苏锐1, 葛庆兰1, 陈宏1, 3, 沈善义2, 雷初朝1   

  1. 1. 西北农林科技大学动物科技学院, 杨凌 712100
    2. 山东天龙驴产业研究院, 无棣 251900
    3. 徐州师范大学细胞与分子生物学研究所, 徐州 221116

  • 收稿日期:2007-08-10 修回日期:2007-11-03 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 雷初朝

African origin of Chinese domestic donkeys

LU Chang-Ji1, XIE Wen-Mei1, SU Rui1, GE Qing-Lan1, CHEN Hong1, 3, SHEN Shan-Yi2, LEI Chu-Zhao1
  

  1. 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China; 2. Shandong Tianlong Academy of Donkey, Wudi 251900, China;
    3. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China
  • Received:2007-08-10 Revised:2007-11-03 Online:2008-03-10 Published:2008-03-10
  • Contact: LEI Chu-Zhao

摘要:

对我国13个家驴品种367条序列(其中引用文献资料241条)的mtDNA D-loop 区399 bp进行分析, 共检测到96种单倍型57个多态位点, 其单倍型多样度为0.767~0.967, 核苷酸多样度为0.014~0.032, 表明我国家驴的遗传多态性丰富。与3个努比亚野驴、3个索马里野驴和6个亚洲野驴的序列构建 NJ系统发育树, 证明我国家驴的母系起源为非洲野驴中的索马里驴和努比亚驴, 亚洲野驴不是中国家驴的母系祖先。

关键词: 遗传多样性, 起源, mtDNA D-loop, 中国家驴

Abstract:

Analysis of the 367 mtDNA D-loop sequences (of which 241 sequences were collected from literature) of 399 bp in 13 Chinese domestic donkey breeds revealed 96 different haplotypes with 57 polymorphic sites. The haplotype diversity and the nucleotide diversity were 0.767-0.967 and 0.014-0.032, respectively, indicating abundant genetic diversity in Chinese domestic donkeys. The Neighbor-joining tree of Chinese domestic donkey sequences was constructed with 3 Nubian wild ass sequences, 3 Somali wild ass sequences and 6 Asian wild ass sequences. Our results suggest that the maternal ancestor of Chinese domestic donkeys is highly likely to be Somali and Nubian of African wild ass instead of Asian wild ass.