遗传 ›› 2009, Vol. 31 ›› Issue (4): 381-386.doi: 10.3724/SP.J.1005.2009.00381

• 研究报告 • 上一篇    下一篇

西北汉族人群D10S1248、D2S441、D1S1677、D9S1122、D10S1435等5个miniSTR基因座的遗传多态性及其法医学应用

白雪;丛斌;李淑瑾;郭霞;李霞
  

  1. 河北医科大学法医学系, 河北省法医学重点实验室, 石家庄050017
  • 收稿日期:2008-09-09 修回日期:2008-11-11 出版日期:2009-04-10 发布日期:2009-04-10
  • 通讯作者: 丛斌

Polymorphism and forensic applications of miniSTR loci D10S1248, D2S441, D1S1677, D9S1122, and D10S1435 in Northwestern Han population

BAI Xue;CONG Bin;LI Shu-Jin;GUO Xia, LI Xia
  

  1. Department of Forensic Biology, Hebei Medical University, Shijiazhuang 050017, China
  • Received:2008-09-09 Revised:2008-11-11 Online:2009-04-10 Published:2009-04-10
  • Contact: CONG Bin

摘要: 为了调查D10S1248、D2S441、D1S1677、D9S1122、D10S1435等5个miniSTR(mini short tandem repeats)基因座在西北汉族人群中的遗传多态性、遗传稳定性及其在陈旧降解检材中的法医学应用价值, 文章采用荧光PCR和基因分型技术对西北汉族154份无关个体血样、10个家系血液样本及10份陈旧降解检材进行片段长度分析。在西北汉族人群中, 5个miniSTR基因座分别检测出了8、7、7、6、7个等位基因, 等位基因频率分布符合Hardy-Weinberg平衡定律, 杂合度(Heterozygosity, H)为0.662~0.792, 个人识别率(Power of discrimination, PD)为0.869~0.915, 非父排除率(Power of exclusion, PE)为0.382~0.585, 多态信息含量(Polymorphism informa-tion content, PIC)为0.650~0.750。家系和陈旧降解检材的研究表明, 5个miniSTR基因座具有高度的遗传稳定性, 可对陈旧降解检材DNA进行有效的分型。5个miniSTR基因座适合作为西北汉族人群的遗传标记, 用于陈旧降解检材的法医学个人识别和亲权鉴定案件中。

关键词: 基因频率, miniSTR, 西北汉族, 遗传多态性

Abstract: To investigate the polymorphism, genetic stability and the value for analysis degraded samples of D10S1248/ D2S441/D1S1677/D9S1122/D10S1435 five loci in northwestern Han population. The lengths of fragments were analyzed by fluorescence PCR and ABI310 Genetic Analyzer. Samples from 154 unrelated northwestern Han individuals, 10 gene-alogies and 10 highly degraded specimens were genotyped. Among the 154 unrelated northwestern Han individuals, we discovered 8, 7, 7, 6, and 7 alleles in the five miniSTR loci, respectively. The frequency distributions in the loci showed no deviations from Hardy–Weinberg equilibrium expectations. The H is at 0.662−0.792, PD is at 0.869−0.915, PE is at 0.382−0.585, and PIC is at 0.650−0.750.The research of genetic stability and the value for analysis degraded sample indi-cated that the five miniSTR loci had high genetic stability and could analysis the degraded samples effectively. Therefore, these 5 miniSTR loci can be used as genetic markers of Northwestern Han populations in forensic practice involving indi-vidual identification and paternity testing from degraded DNA samples.