遗传 ›› 2005, Vol. 27 ›› Issue (6): 882-886.

• 研究报告 • 上一篇    下一篇

山西猪种及其杂种群体H-FABP基因的PCR-RFLP研究

杨文平1 ;曹果清1 ;石建忠2 ;刘建华1;李步高1 ;周忠孝1   

  1. 山西农业大学动物科技学院, 太谷 030801
  • 收稿日期:2004-08-22 修回日期:2005-02-22 出版日期:2005-12-10 发布日期:2005-12-10
  • 通讯作者: 杨文平

Research on the Polymorphism of Heart Fatty Acid-binding Protein Gene in Shanxi Pig Breeds and Their Crossbred Populations Using PCR-RFLP

YANG Wen-ping1,CAO Guo-qing1,SHI Jian-zhong2,LIU Jian-hua1,LI Bu-gao1,ZHOU Zhong-xiao1   

  1. College of Animal Science and Technology ,Shanxi Agricultural University,Taigu 030801,China
  • Received:2004-08-22 Revised:2005-02-22 Online:2005-12-10 Published:2005-12-10

摘要: 利用PCR-RFLP技术对马身猪、山西白猪及其杂种群体共286头猪的心脏脂肪酸结合蛋白(H-FABP)基因5′-上游区(HinfⅠ-RFLP)和第二内含子内(HinfⅠ*-RFLP和Hae Ⅲ-RFLP)的遗传变异进行了研究。结果表明:(1)在Hae Ⅲ-RFLP位点上,马身猪均为DD纯合子,而其他猪群在此位点上均存在变异,马身猪的杂种群体在该位点上只有两种基因型(DD、Dd);(2)在5′-上游区的HinfⅠ-RFLP位点上,杜洛克猪×山西白猪的杂种群体只有HH基因型,而其他群体都表现出多态性,马身猪等位基因h的频率为0.9727;(3)在第二内含子内的HinfⅠ*-RFLP位点上,马身猪表现出两种基因型(BB、Bb),等位基因B的频率为0.9667;(4)在HinfⅠ*-RFLP和Hae Ⅲ-RFLP位点上,所有猪群均处于Hardy –Weinberg 平衡状态。

关键词: H-FABP基因, PCR-RFLP, 猪, 多态性

Abstract: The genetic variation in 5′- upstream (HinfⅠ-RFLP)and the second intron (HinfⅠ*-RFLP、Hae Ⅲ-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen,Shanxi white pig and their crossbred populations. The results showed as follows:(1)Mashen presented only DD genotype while other populations varied,and Mashen crossbred populations had only 2 genotypes(DD、Dd)at the Hae Ⅲ-RFLP site;(2)At the HinfⅠ-RFLP site of the 5′-upstream region,the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. (3)At the HinfⅠ*-RFLP site of the second intron ,only Mashen presented 2 genotypes(BB、Bb),and frequency of B allele was 0.9667.(4)At the Hae Ⅲ-RFLP and HinfⅠ*-RFLP sites,all populations were in Hardy-weinberg equilibrium.

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